DOI: 10.1128/AEM.02289-10
ABSTRACT
The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange.
FOOTNOTES
- Received 24 September 2010.
- Accepted 8 April 2011.
- Accepted manuscript posted online 29 April 2011.
↵† Supplemental material for this article may be found at http://dx.doi.org/10.1128/AEM.02289-10.
- Copyright © 2011, American Society for Microbiology. All Rights Reserved.
