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Methods

Continuous Monitoring of Ammonia Removal Activity and Observation of Morphology of Microbial Complexes in a Microdevice

Kensuke Toda, Yutaka Yawata, Erika Setoyama, Junji Fukuda, Nobuhiko Nomura, Hiroaki Suzuki
Kensuke Toda
1Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan,
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Yutaka Yawata
2 Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan
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Erika Setoyama
2 Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan
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Junji Fukuda
1Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan,
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Nobuhiko Nomura
2 Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan
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Hiroaki Suzuki
1Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan,
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  • For correspondence: hsuzuki@ims.tsukuba.ac.jp
DOI: 10.1128/AEM.01246-10
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    Fig. 1.

    Microdevice for continuous monitoring of microbial activity. (A) Top view of the device. The dimensions of the chip are 18 mm by 18 mm. (B) Cross-section along the line X-X' in panel A. (C) Dependence of the potential of the ion-selective electrode on NH4+ ion concentration. Potential of Ag/AgCl(1) with respect to Ag/AgCl(2) was measured. Five measurements were made, and averages and standard deviations are shown. Many of the error bars are behind the symbols.

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    Fig. 2.

    Continuous monitoring of the ammonia removal activity and observation of the morphology of the active sludge. (A) Changes in the NH4+ ion concentration in the medium with or without the active sludge. The response curves in the medium with the active sludge were obtained by three independent experiments. The insets are representative images obtained using confocal reflection microscopy at the indicated times. Each projection shows fields of 140 by 140 by 30 μm3 (depth × width × height). The thickness of each image stack was 0.8 μm. (B) LIVE/DEAD fluorescent staining at 0 and 48 h of culture. Scale bars indicate 40 μm.

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Continuous Monitoring of Ammonia Removal Activity and Observation of Morphology of Microbial Complexes in a Microdevice
Kensuke Toda, Yutaka Yawata, Erika Setoyama, Junji Fukuda, Nobuhiko Nomura, Hiroaki Suzuki
Applied and Environmental Microbiology Jun 2011, 77 (12) 4253-4255; DOI: 10.1128/AEM.01246-10

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Continuous Monitoring of Ammonia Removal Activity and Observation of Morphology of Microbial Complexes in a Microdevice
Kensuke Toda, Yutaka Yawata, Erika Setoyama, Junji Fukuda, Nobuhiko Nomura, Hiroaki Suzuki
Applied and Environmental Microbiology Jun 2011, 77 (12) 4253-4255; DOI: 10.1128/AEM.01246-10
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