SidL, an Aspergillus fumigatus Transacetylase Involved in Biosynthesis of the Siderophores Ferricrocin and Hydroxyferricrocin

ABSTRACT The opportunistic fungal pathogen Aspergillus fumigatus produces four types of siderophores, low-molecular-mass iron chelators: it excretes fusarinine C (FsC) and triacetylfusarinine C (TAFC) for iron uptake and accumulates ferricrocin (FC) for hyphal and hydroxyferricrocin (HFC) for conidial iron distribution and storage. Siderophore biosynthesis has recently been shown to be crucial for fungal virulence. Here we identified a new component of the fungal siderophore biosynthetic machinery: AFUA_1G04450, termed SidL. SidL is conserved only in siderophore-producing ascomycetes and shows similarity to transacylases involved in bacterial siderophore biosynthesis and the N 5-hydroxyornithine:anhydromevalonyl coenzyme A-N 5-transacylase SidF, which is essential for TAFC biosynthesis. Inactivation of SidL in A. fumigatus decreased FC biosynthesis during iron starvation and completely blocked FC biosynthesis during iron-replete growth. In agreement with these findings, SidL deficiency blocked conidial accumulation of FC-derived HFC under iron-replete conditions, which delayed germination and decreased the size of conidia and their resistance to oxidative stress. Remarkably, the sidL gene is not clustered with other siderophore-biosynthetic genes, and its expression is not affected by iron availability. Tagging of SidL with enhanced green fluorescent protein suggested a cytosolic localization of the FC-biosynthetic machinery. Taken together, these data suggest that SidL is a constitutively active N 5-hydroxyornithine-acetylase required for FC biosynthesis, in particular under iron-replete conditions. Moreover, this study revealed the unexpected complexity of siderophore biosynthesis, indicating the existence of an additional, iron-repressed N 5-hydroxyornithine-acetylase.

Aspergillus fumigatus is a ubiquitous saprophytic mold but also the most common cause of airborne fungal infections in immunocompromised patients. The incidence of invasive Aspergillus infections, termed aspergillosis, increased enormously in recent decades, with a mortality rate ranging from 60 to 90% (35). During both saprophytic and pathogenic growth, this opportunistic pathogen has to cope with rapidly changing microenvironments. Therefore, A. fumigatus is able to adapt its metabolism in order to overcome nutrient sequestration and host defense mechanisms. Virtually all organisms require iron as an indispensable cofactor for various metabolic processes. The biological importance of iron is attributed largely to its chemical properties as a transition metal, which allow oneelectron oxidation-reduction reactions between its ferric and ferrous states. Paradoxically, this capacity also explains why iron excess causes oxidative damage of macromolecules and cell membranes, because iron can act as a catalyst in Haber-Weiss/Fenton chemistry (11). Therefore, cellular iron homeostasis has to combine sufficient iron supply with prevention of iron toxicity. Siderophores (low-molecular-mass chelators of ferric iron) play a central role in the iron metabolism of most bacteria and fungi. Aspergillus fumigatus produces four siderophores: fusarinine C (FsC), triacetylfusarinine C (TAFC), ferricrocin (FC), and hydroxyferricrocin (HFC). FsC and TAFC are excreted for the solubilization and uptake of iron (29); FC is used for hyphal iron storage and distribution, and HFC is required for conidial iron storage. Due to the role of FC in intracellular iron storage and distribution, its biosynthesis plays a crucial role in germination and conidiation (29,36). In Aspergillus nidulans, FC has also been shown to be essential for sexual development (7). In addition to siderophore-mediated iron uptake, A. fumigatus employs a second high-affinity iron uptake system, reductive iron assimilation, as well as low-affinity iron uptake but is incapable of directly utilizing host iron sources such as heme, ferritin, and transferrin (28,29). A lack of extracellular siderophores causes oxidative stress because it increases iron starvation, and iron is essential for various metabolic functions, in particular for the activity of antioxidative enzymes, such as the heme-containing catalase (29). Intracellular siderophores also play a crucial role in the prevention of oxidative stress due to their role in intracellular iron supply and because chelation by siderophores renders iron Fenton-inactive (31).
Previously, both siderophore biosynthesis and reductive iron assimilation have been found to be induced at the transcriptional level during infection in a murine model of pulmonary aspergillosis (27,29). However, biosynthesis of both extracellular and intracellular siderophores, but not reductive iron assimilation, proved essential for full virulence of Aspergillus fumigatus in a murine model of pulmonary aspergillosis (28,29). In later studies, siderophore biosynthesis proved crucial for virulence in a murine cutaneous model, in Drosophila melanogaster, and in Galleria mellonella (2,5,33). In the murine host, siderophore biosynthesis was found to be important for extracellular as well as intracellular growth (32). Moreover, extracellular and intracellular siderophore biosynthesis has also been shown to be crucial for the virulence of plant-pathogenic ascomycetes (9,13,22,23).
Previous studies identified five A. fumigatus genes required for the biosynthesis of siderophores (Fig. 1). The biosynthesis of all four Aspergillus siderophores depends on the hydroxylation of ornithine, catalyzed by the monooxygenase SidA (12,28). For the synthesis of FsC and TAFC, hydroxyornithine is acylated by SidF to yield anhydromevalonyl-hydroxyornithine, which is used by the nonribosomal peptide synthetase (NRPS) SidD to generate cyclic FsC (29). FsC is acetylated by SidG to TAFC (29). For FC and HFC synthesis, hydroxyornithine requires acetylation by an as yet unknown enzyme. Three acetylhydroxyornithine molecules are coupled to one serine and two glycine residues by the nonribosomal peptide synthetase SidC to yield cyclic FC (29). FC is hydroxylated to HFC by an as yet uncharacterized enzyme during conidiation (10,29). The expression of all A. fumigatus siderophore-biosynthetic genes identified so far is subject to transcriptional repression by iron, which is mediated in part by the GATA-type transcription factor SreA (31). Hydroxylation of FC to HFC is developmentally regulated (29). Moreover, extracellular and intracellular siderophore biosynthesis is positively affected by the bZip transcription factor HapX (27). Most fungal species produce siderophores and possess orthologs to most of the A. fumigatus siderophore-biosynthetic genes (10). In contrast, fungal species lacking siderophores, such as Saccharomyces cerevisiae, Candida albicans, or Cryptococcus neoformans, lack such orthologs.
In the present study, we report the identification and functional characterization of one of the missing siderophore-biosynthetic genes. We demonstrate that the GCN5-related N-acetyltransferase (GNAT)-type acetyltransferase AFUA_1G04450, termed SidL, is essential for the biosynthesis of FC and HFC during iron-replete growth. Thus, the constitutively expressed sidL gene encodes the first fungal N 5 -hydroxyornithine:acetyl coenzyme A (CoA)-N 5 -transacylase identified so far. FC production by a sidL deletion mutant during iron starvation indicates the existence of a second N 5 -hydroxyornithine:acetyl-CoA-N 5 -transacylase, the expression or activation of which is repressed by iron.

MATERIALS AND METHODS
Growth conditions. All strains used in this study were grown at 37°C in Manipulation of nucleic acids and strain construction. Standard molecular techniques were performed as described by Sambrook et al. (26), using the pGEM-T vector system (Promega) and the bacterial strain Escherichia coli DH5␣ cultivated in LB medium (1% Bacto tryptone, 0.5% yeast extract, 1% NaCl [pH 7.5]). The strains used in this study are listed in Table 1.
To generate a sidL deletion mutant, a 3.7-kb fragment comprising the sidL coding region, a 1.2-kb 5Ј upstream region, and a 1.1-kb 3Ј downstream region was PCR amplified from genomic A. fumigatus ATCC 46645 DNA using oligonucleotides sidL3 and sidL6, and the product was inserted into the pGEM-T plasmid (Promega), yielding psidL. The 2-kb EcoRI/SmaI fragment comprising the entire sidL coding region was replaced by a 2.0-kb MfeI/XhoI (blunt-ended) fragment of plasmid pSK275 (16,17) carrying the pyrithiamine selection marker cassette, yielding plasmid psidLko. The gel-purified 3.6-kb NciI/ScaI fragment of psidLko was used for the transformation of ATCC 46645. A. fumigatus was transformed as described previously (25,31). Pyrithiamine-resistant sidL deletants (the ⌬sidL strain) were used for further analysis.
In order to complement the ⌬sidL strain, a 4.4-kb fragment containing the sidL gene was PCR amplified from genomic DNA using oligonucleotides sidL9 and sidL6. The PCR product was inserted into the ⌬sidL strain via cotransformation with plasmid pAN7.1, conferring resistance to hygromycin (25). Hygromycin-resistant transformants with homologous reconstitution of the sidL gene (the sidL c strain) were selected by PCR (data not shown) and Southern blot analysis. The hybridization probe for Southern blot analysis of the ⌬sidL and sidL c strains was generated by PCR using oligonucleotides sidL3 and sidL7 and optrA1 and prtA2, respectively.
In order to localize SidL subcellularly, an in-frame egfp (enhanced green fluorescent protein-encoding gene)-sidL-fusion under the control of the constitutive XAH promoter was generated (8). For this purpose, the sidL-coding region was amplified from A. fumigatus genomic DNA using oligonucleotides sidL-gfp1-BglII and sidL-gfp2-NotI, which carry the restriction enzyme sites BglII and NotI, respectively. Subsequently, the HapX-encoding BglII/NotI fragment of plasmid pCAME703 was replaced by the SidL-encoding BglII/NotI fragment, yielding plasmid psidLgfp. Plasmid psidLgfp was inserted into the ⌬sidL strain via cotransformation with plasmid pAN8.1 (25), conferring resis- tance to phleomycin. The egfp-sidL fusion version could restore the wild-type (wt) phenotype. Homokaryotic transformants were obtained by picking colonies originating from single spores. Correct gene deletion was confirmed by PCR (data not shown) and Southern blot analysis (see Fig. S1 in the supplemental material). A. fumigatus DNA was isolated as described previously (37). The sequences of the oligonucleotides used in this study are listed in Table S1 in the supplemental material.
Analysis of siderophore production. Extracellular and intracellular siderophores were isolated as described by Oberegger et al. (21), and siderophore analysis was carried out by reversed-phase high-performance liquid chromatography (HPLC) analysis according to the method of Konetschny-Rapp et al. (15), as described previously (21). For determination of conidial HFC contents, fungal strains were cultured in either iron-replete or iron-depleted liquid AMM for 18 h, and then mycelia were transferred to iron-replete plates for sporulation. After 4 days, spores were harvested and lyophilized, and HFC levels were determined.
Northern blot analysis. RNA was isolated using the TRI reagent (Sigma-Aldrich). Fifteen micrograms of total RNA was used for electrophoresis on 1.2% agarose-2.2 M formaldehyde gels and was blotted onto Hybond N membranes (Amersham Biosciences). The probes used in this study were generated by PCR with the digoxigenin labeling system (Roche Molecular Biochemicals).
Determination of germination and conidial size. For the determination of germination capacity, 10 4 conidia were incubated at 37°C for 13 h on 6-well chambered cover glasses filled with 3 ml AMM. The germination of 40 spores for each strain was scored in triplicate by light microscopy (Zeiss Axioplan); images were acquired with an AxioCam MRc digital camera (Zeiss) and were processed with Adobe Photoshop, version 7.0.
For determination of spore sizes, the diameters of 30 freshly harvested conidia in 0.9% NaCl-0.01% Tween 80 were determined from three biological triplicates by light microscopy (see above). For scanning electron microscopy, strains were grown on AMM plates at 25°C for 4 days and were fixed with 2.5% glutardialdehyde in 0.1 M phosphate buffer (pH 7.3). A brief wash in the same buffer was followed by 1 h of postfixation with 1% aqueous OsO 4 , gradual dehydration with ethanol, and critical-point drying with liquid CO 2 (CPD 030 critical-point dryer; Bal-Tec AG, Balzers, Liechtenstein). Specimens were mounted on aluminum stubs with Leit C (Göcke, Plano GmbH), sputtered with 10 nm Au-Pd (MED 020 sputtering device; Bal-Tec AG, Balzers, Liechtenstein), and examined with a Zeiss field emission scanning electron microscope (FESEM; Gemini 982).
Oxidative-stress sensitivity. Susceptibility to reactive oxygen species was determined as described previously (34). A total of 10 7 A. fumigatus conidia were mixed with 10 ml AMM top agar and were poured onto AMM plates. In the middle of the plate, a 5-mm-diameter hole was pricked out and filled with 100 l of a 100 mM H 2 O 2 solution. After incubation for 24 h at 37°C, the diameter of the growth inhibition zone was measured.
Phylogenetic analysis. A BLASTP search was carried out with the PSI-BLAST algorithm in the NCBI database to look for putative SidL homologs (1). A ClustalW alignment was done with the Pasteur bioweb2 database, using selected sequences with E values higher than 5eϪ10 in the PSI-BLAST search. Based on this alignment, a rooted tree was generated using the Pasteur bioweb2 database (18) (http://mobyle.pasteur.fr).

RESULTS AND DISCUSSION
SidL, a putative transacylase involved in siderophore biosynthesis. Biosynthesis of FC and HFC involves acetylation of N 5 -hydroxyornithine to N 5 -acetyl-N 5 -hydroxyornithine. No genes encoding this enzymatic activity have been identified in fungi yet. In bacteria, however, genes coding for enzymes catalyzing similar reactions have been identified. These enzymes contain the ϳ45-amino-acid pfam10331/AlcB domain and belong to the GNAT (GCN5-related N-acetyltransferase) superfamily, including functionally diverse enzymes that catalyze the transfer of an acetyl group from acetyl-CoA to the primary amine of a wide range of acceptor substrates. Mem-bers of the GNAT superfamily include aminoglycoside Nacetyltransferases, serotonin N-acetyltransferase, glucosamine-6-phosphate N-acetyltransferase, histone acetyltransferases, mycothiol synthase, and the Fem family of aminoacyl transferases. Examples of siderophore-biosynthetic enzymes of the pfam10331/AlcB domain-containing subfamily include Bordetella bronchiseptica AlcB, Escherichia coli IucB, and Mycobacterium tuberculosis Rv1347c (4, 3, 6). AlcB appears to catalyze the N-acylation of the hydroxylamine group in N-hydroxyputrescine with succinyl-CoA in alcaligen biosynthesis; IucB and Rv1347c are N 6 -hydroxylysine:acetyl-CoA-N 6 -transacetylases involved in the biosynthesis of aerobactin and mycobactin, respectively.
Further BLASTP searches and phylogenetic analysis revealed the presence of SidL and SidF orthologs in siderophoreproducing species (but not in non-siderophore-producing fungal species, such as Saccharomyces cerevisiae, Candida albicans, or Cryptococcus neoformans), whereby SidL and SidF orthologs form clear subclades ( Fig. 2A).
In contrast to A. fumigatus SidL, most fungal orthologs contain a highly conserved C-terminal extension of about 32 amino acid residues, e.g., Neosartorya fischeri XP_001265221 and Aspergillus clavatus XP_001269742. Reverse transcription-PCR (RT-PCR) analysis revealed that A. fumigatus sidL contains an intron, which was not predicted by the genome annotation, at the very same position as that in the fungal orthologs (data not shown). The reannotated SidL sequence now also contains the conserved C terminus (Fig. 2B).
Except for sidA and npgA, all known siderophore-biosynthetic genes of A. fumigatus, including sidF, are organized in gene clusters (10,20,31). In contrast to the sidA product, the npgA-encoded phosphopantetheinyl transferase NpgA is not devoted solely to the biosynthesis of siderophores but is required for all metabolites depending on nonribosomal peptide synthetases and polyketide synthases (14,20). The sidL gene is not clustered with other known siderophore-biosynthetic genes: it is flanked by genes encoding a putative PrnX-like proline utilization protein (AFUA_1G04440) and a putative lysyl-tRNA synthetase (AFUA_1G04460).
Expression of sidL is regulated neither by iron availability nor by the iron regulator SreA. With the exception of conidial HFC, the production of all A. fumigatus siderophores is repressed by iron (10). Accordingly, the expression of all genes identified so far encoding siderophore-biosynthetic enzymes is transcriptionally repressed by iron, and the repression is mediated, at least in part, by the GATA-type transcriptional repressor SreA (10,31). The only exception is the constitutively expressed npgA gene (20), which is, however, not dedicated solely to siderophore metabolism, as mentioned above. More- over, FC production is additionally upregulated by intracellular iron excess caused by inactivation of SreA, which results in derepressed iron uptake under iron-replete conditions. To investigate possible regulation by iron or SreA, we analyzed the sidL transcript levels in ATCC 46645 (wt) and in the SreA-deficient ⌬sreA mutant under iron-replete and iron-depleted conditions by Northern blot analysis (Fig. 3). Neither iron availability nor SreA deficiency affected the levels of sidL transcripts, in contrast to the known SreA target mirB (31). In agreement with this finding, the sidL promoter region lacks the predicted SreA consensus binding motif ATCWGATAA (31).
SidL is involved in the biosynthesis of FC and HFC. In order to functionally characterize SidL, the sidL coding region was replaced by the pyrithiamine resistance marker gene ptrA in A. fumigatus ATCC 46645 as described in Materials and Methods, yielding the ⌬sidL strain (see Fig. S1 in the supplemental material). To ensure characterization of gene deletion-specific effects, a single functional copy of the sidL strain was homologously integrated at the resident sidL locus in the ⌬sidL strain, yielding the sidL c strain (see Fig. S1). In all assays, the reconstituted sidL c strain showed wt characteristics (data not shown). Northern blot analysis revealed wt-like expression of the sidL-neighboring genes AFUA_1g04440 and AFUA_1g04460 in the ⌬sidL strain (see Fig. S2 in the supplemental material). Furthermore, PCR amplification and sequencing of the genomic region of the ⌬sidL strain used for homologous integration of the sidL deletion construct confirmed the wt sequence (data not shown). Taken together, these data support the idea that the ⌬sidL phenotype is indeed caused by inactivation of SidL function.
Based on the similarity of SidL to acyltransferases involved in siderophore biosynthesis, we analyzed the effects of sidL deletion on extracellular and intracellular siderophore production after 24 h and 48 h under iron-depleted and iron-replete conditions (Fig. 4A). As reported previously (29,31), extracellular TAFC production was detected only during iron starvation, not during iron sufficiency, and was 1.25-fold Ϯ 0.03-fold higher in the ⌬sidL strain than in the wt (discussed below). As shown previously (29,31) (Fig. 2), the sidL expression profile (Fig. 3), and the effects of sidL deletion on FC biosynthesis (Fig. 4A) indicate that sidL encodes a constitutively expressed N 5 -hydroxyornithine:acetyl-CoA-N 5transacetylase required for the biosynthesis of FC, in particular under iron-replete conditions. Moreover, the data demonstrate that A. fumigatus possesses a second N 5 -hydroxyornithine:acetyl-CoA-N 5 -transacetylase involved in the biosynthesis of FC, the expression or activity of which is upregulated during iron starvation. This enzyme must belong to a different protein family, since the A. fumigatus genome does not encode a SidL homolog apart from SidF (19).
For conidial iron storage, A. fumigatus employs HFC, which is derived from FC by hydroxylation. HFC production is induced by conidiogenesis, i.e., it is developmentally regulated (29). Consistent with its blockage of FC production under iron-replete conditions, SidL deficiency also blocked HFC storage in conidia harvested after iron-replete growth (Fig. 4B). In contrast, conidia harvested from ⌬sidL mycelia grown under conditions of iron starvation and then shifted to iron-replete conditions before sporulation showed a wt-like HFC content (Fig. 4B). This indicates that the unknown iron starvation-induced N 5 -hydroxyornithine: acetyl-CoA-N 5 -transacetylase can compensate for the loss of SidL. The loss of conidial HFC in the ⌬sidL strain can be cured by supplementation with FC (Fig. 4D), which is taken up by the hyphae, hydroxylated, and transported into the conidia, as shown previously for other (⌬sidA and ⌬sidC) FC-deficient mutants (29,36).
In A. fumigatus, iron starvation causes a massive remodeling of the cellular free amino acid pool, including a 7-fold increase in levels of the siderophore precursor ornithine (27). Comparison of the amino acid pool compositions of the ⌬sidL and wt Pseudomonas aeruginosa; Psp, Pseudomonas putida; Re, Ralstonia eutropha; Sp, Schizosaccharomyces pombe; Um, Ustilago maydis. (B) The sequence numbering refers to SidL. Invariant residues (white letters on a black background) and residues conserved in three of the four sequences (shaded) are indicated. A. fumigatus SidL displays 43% sequence identity to E. coli IucB. The PSI-BLAST homology search with SidL revealed E values of 2eϪ35, 2eϪ39, and 3eϪ11 for IucB, SidF, and RV1347c, respectively. strains under iron-replete and iron-depleted conditions revealed no significant differences (data not shown). Therefore, inactivation of SidL-mediated consumption of ornithine for FC biosynthesis does not result in cellular accumulation of ornithine. The ⌬sidL strain displayed 1.25-fold-higher levels of TAFC biosynthesis than the wt during iron starvation, indicating that the N 5 -hydroxyornithine not consumed by SidL is shuttled into TAFC biosynthesis. SidL deficiency results in reduced conidiation, conidial iron starvation, reduced conidial size, reduced growth during iron starvation, delayed germination, and increased oxidativestress sensitivity. FC is involved in both the intracellular storage and the distribution of iron in A. nidulans and A. fumigatus and is therefore crucial for optimal conidiation, conidial iron homeostasis, oxidative-stress resistance, and germination (7,36).
Like the FC-deficient ⌬sidC mutant (7,29), the ⌬sidL strain displayed wt-like conidiation when supplemented with FC (which cures FC deficiency, as shown above) but a 15% decrease in the production of conidia under iron-replete condi-tions (data not shown), most probably due to reduced intracellular iron distribution (36).
Northern blot analysis revealed downregulation of the ironinduced sreA gene and upregulation of the iron-repressed hapX gene in ⌬sidL conidia harvested from iron-replete plates relative to expression in the wt (Fig. 5A). Reconstitution of the conidial HFC content by FC supplementation during sporulation cured this ⌬sidL defect, supporting the notion that the loss of HFC is responsible for this ⌬sidL defect (Fig. 5A). These data indicate that ⌬sidL conidia are iron starved due to a reduced iron supply during conidiogenesis caused by FC/HFC deficiency, as found previously for conidia of the FC-deficient A. fumigatus ⌬sidA and ⌬sidC mutant strains (30).
Light microscopy and scanning electron microscopy revealed that SidL deficiency reduces the size of conidia harvested from iron-replete plates by 13% (3.2 Ϯ 0.3 m for the wt and 2.8 Ϯ 0.2 m for the ⌬sidL strain; P, Ͻ0.0001 by a paired two-tailed t test). Analysis of ⌬sidA and ⌬sidC conidia displayed similar reductions in conidial sizes (data not shown), supporting the notion that this defect is caused by FC defi- ciency. In agreement with this finding, FC supplementation cured this defect (data not shown), which is most likely caused by iron starvation during conidiogenesis or within conidia (see above). The ⌬sidL strain displayed reduced growth during iron starvation, but this defect was cured by reconstitution of the conidial HFC content by FC supplementation during conidiation (Fig. 5B). These data indicated a germination defect. Accordingly, ⌬sidL conidia but not HFC-reconstituted ⌬sidL conidia displayed delayed germination, in particular under iron-depleted conditions (Fig. 5C), confirming the notion that the conidial FC-iron content supports germination, as demonstrated previously for A. nidulans and A. fumigatus ⌬sidA and ⌬sidC mutant strains (7,29).
To analyze the putative role of SidL in oxidative-stress resistance, wt and ⌬sidL conidia derived from media with or without FC supplementation were exposed to hydrogen peroxide in a plate diffusion assay as described in Materials and Methods. HFC-deficient ⌬sidL conidia from high-iron plates displayed increased hydrogen peroxide sensitivity (inhibition zone, 20.3 Ϯ 0.4 mm for the ⌬sidL strain compared to 13.4 Ϯ 0.5 mm for the wt). Reconstitution of the HFC content of the ⌬sidL strain by FC supplementation largely cured this defect (inhibition zones, 11.2 Ϯ 0.3 and 9.6 Ϯ 0.4 mm for the wt and ⌬sidL strains, respectively). These data demonstrate that SidLmediated production of FC and HFC is crucial for conidial resistance to oxidative stress. In agreement, increased oxidative-stress sensitivity has been reported previously for the FC/ HFC-deficient ⌬sidA and ⌬sidC mutant strains of A. nidulans and A. fumigatus (7,29). The decreased oxidative-stress resistance of HFC-deficient conidia is most likely due to the resulting iron starvation, which impairs iron-dependent antioxidative functions, such as the heme-requiring catalase A (36). Remarkably, these data also revealed that ferricrocin supplementation slightly increases the oxidative-stress resistance of the wt (inhibition zones, 13.4 Ϯ 0.5 mm and 11.2 Ϯ 0.3 mm without and with FC supplementation), presumably due to improved iron supply.
SidL is localized in the cytoplasm. To date, the subcellular localization of siderophore biosynthesis has been unknown. To clarify the localization of the FC/HFC-biosynthetic machinery, the 5Ј end of sidL was fused in frame with the 3Ј end of the EGFP-encoding gene expressed under the control of a constitutive promoter, yielding the egfp-sidL strain (see Fig. S1 in the supplemental material). Analysis of the hyphal and conidial siderophore contents and determination of the growth rate of the egfp-sidL strain (data not shown) demonstrated that the N-terminally EGFP tagged SidL protein is biologically functional. Epifluorescence microscopy revealed that SidL is localized in the cytoplasm (Fig. 6).
Conclusions. This study identified a novel component of the fungal siderophore-biosynthetic machinery, the cytosolic N 5hydroxyornithine:acetyl-CoA-N 5 -transacetylase involved in FC biosynthesis. The sidL gene encoding this enzyme represents an exception, in that it is the first siderophore-biosynthetic gene not to be regulated by iron availability. Like sidA but unlike the other siderophore-biosynthetic genes identified (31), sidL is not genomically clustered with other siderophorebiosynthetic genes. This study also revealed that A. fumigatus possesses at least two N 5 -hydroxyornithine:acetyl-CoA-N 5transacetylases: constitutively expressed SidL and an unidentified enzyme, the expression or activation of which is repressed by iron. The rationale for a constitutively active N 5 -hydroxyornithineacetylase form might relate to the crucial roles of FC and HFC in intracellular iron distribution and conidiation, functions that are also important under iron-replete conditions. The homology of SidL, SidF, and bacterial siderophore-biosynthetic acyl- transferases indicates the common origin of some components of bacterial and fungal siderophore systems.