Article Figures & Data
Figures
- Fig. 1.
Origin of C atoms in purine and pyrimidine de novo synthesis and 13C labeling by use of [13C]urea as the tracer substance.
- Fig. 2.
13C enrichment of the 16S rRNA of total bacteria (measured using probe EUB338) after 24 h of incubation of 1% human fecal sample with either [13C]glycine (1.5 mg/ml), NaH13CO3 (0.679 mg/ml), or [13C]urea (0.939 mg/ml) in mineral medium with 10 g/liter oligofructose. Values are given as means ± standard deviations; **, P < 0.01.
- Fig. 3.
13C enrichment of the 16S rRNAs of total bacteria (A), the Bacteroides-Prevotella group (B), bifidobacteria (C), the Eubacterium rectale-Clostridium coccoides group (D), and the Clostridium leptum subgroup (E) after 6 h of incubation of 1% human fecal sample with [13C]urea (0.939 mg/ml) in mineral medium without oligofructose (MM − OF) or with 10 g/liter oligofructose (MM + OF) and in Wilkins-Chalgren broth without oligofructose (WC − OF) or with 10 g/liter oligofructose (WC + OF). **, P < 0.01 versus the control (MM − OF); a, P < 0.05 versus MM + OF.
Tables
- Table 1.
Biotinylated oligonucleotide probes targeting 16S rRNA and hybridization temperatures
Probe name Sequence (5′–3′) Target organisms Hybridization temp (°C) Reference EUB338 Biotin-GCTGCCTCCCGTAGGAGT Most eubacteria 46 1 Bac303 Biotin-CCAATGTGGGGGACCTT Bacteroides-Prevotella group 46 17 Bif164 Biotin-CATCCGGCATTACCACCC Bifidobacteria 45 13 EREC482 Biotin-GCTTCTTAGTCARGTACCG Eubacterium rectale-Clostridium coccoides group 50 8 Clep866 Biotin-GGTGGATWACTTATTGTG Clostridium leptum subgroup 40 14
