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Applied and Environmental Microbiology
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Enzymology and Protein Engineering

Synthesis of Fucosyl-N-Acetylglucosamine Disaccharides by Transfucosylation Using α-l-Fucosidases from Lactobacillus casei

Jes�s Rodr�guez-D�az, Rodrigo J. Carbajo, Antonio Pineda-Lucena, Vicente Monedero, Mar�a J. Yebra
Jes�s Rodr�guez-D�az
aLaboratorio de Bacterias L�cticas y Probi�ticos, Departamento de Biotecnolog�a de Alimentos, IATA-CSIC, Valencia, Spain
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Rodrigo J. Carbajo
bStructural Biochemistry Laboratory, Medicinal Chemistry Department, Centro de Investigaci�n Pr�ncipe Felipe, Valencia, Spain
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Antonio Pineda-Lucena
bStructural Biochemistry Laboratory, Medicinal Chemistry Department, Centro de Investigaci�n Pr�ncipe Felipe, Valencia, Spain
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Vicente Monedero
aLaboratorio de Bacterias L�cticas y Probi�ticos, Departamento de Biotecnolog�a de Alimentos, IATA-CSIC, Valencia, Spain
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Mar�a J. Yebra
aLaboratorio de Bacterias L�cticas y Probi�ticos, Departamento de Biotecnolog�a de Alimentos, IATA-CSIC, Valencia, Spain
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DOI: 10.1128/AEM.00229-13
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ABSTRACT

AlfB and AlfC α-l-fucosidases from Lactobacillus casei were used in transglycosylation reactions, and they showed high efficiency in synthesizing fucosyldisaccharides. AlfB and AlfC activities exclusively produced fucosyl-α-1,3-N-acetylglucosamine and fucosyl-α-1,6-N-acetylglucosamine, respectively. The reaction kinetics showed that AlfB can convert 23% p-nitrophenyl-α-l-fucopyranoside into fucosyl-α-1,3-N-acetylglucosamine and AlfC at up to 56% into fucosyl-α-1,6-N-acetylglucosamine.

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Synthesis of Fucosyl-N-Acetylglucosamine Disaccharides by Transfucosylation Using α-l-Fucosidases from Lactobacillus casei
Jes�s Rodr�guez-D�az, Rodrigo J. Carbajo, Antonio Pineda-Lucena, Vicente Monedero, Mar�a J. Yebra
Applied and Environmental Microbiology May 2013, 79 (12) 3847-3850; DOI: 10.1128/AEM.00229-13

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Synthesis of Fucosyl-N-Acetylglucosamine Disaccharides by Transfucosylation Using α-l-Fucosidases from Lactobacillus casei
Jes�s Rodr�guez-D�az, Rodrigo J. Carbajo, Antonio Pineda-Lucena, Vicente Monedero, Mar�a J. Yebra
Applied and Environmental Microbiology May 2013, 79 (12) 3847-3850; DOI: 10.1128/AEM.00229-13
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