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Plant Microbiology

The Type III Secretion System of Bradyrhizobium japonicum USDA122 Mediates Symbiotic Incompatibility with Rj2 Soybean Plants

Takahiro Tsukui, Shima Eda, Takakazu Kaneko, Shusei Sato, Shin Okazaki, Kaori Kakizaki-Chiba, Manabu Itakura, Hisayuki Mitsui, Akifumi Yamashita, Kimihiro Terasawa, Kiwamu Minamisawa
Takahiro Tsukui
aGraduate School of Life Sciences, Tohoku University, Sendai, Japan
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Shima Eda
aGraduate School of Life Sciences, Tohoku University, Sendai, Japan
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Takakazu Kaneko
bFaculty of Engineering, Kyoto Sangyo University, Kyoto, Japan
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Shusei Sato
cKazusa DNA Research Institute, Kisarazu, Chiba, Japan
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Shin Okazaki
dFaculty of Agriculture, Tokyo University of Agricultural Technology, Tokyo, Japan
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Kaori Kakizaki-Chiba
aGraduate School of Life Sciences, Tohoku University, Sendai, Japan
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Manabu Itakura
aGraduate School of Life Sciences, Tohoku University, Sendai, Japan
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Hisayuki Mitsui
aGraduate School of Life Sciences, Tohoku University, Sendai, Japan
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Akifumi Yamashita
aGraduate School of Life Sciences, Tohoku University, Sendai, Japan
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Kimihiro Terasawa
aGraduate School of Life Sciences, Tohoku University, Sendai, Japan
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Kiwamu Minamisawa
aGraduate School of Life Sciences, Tohoku University, Sendai, Japan
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DOI: 10.1128/AEM.03297-12
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ABSTRACT

The rhcJ and ttsI mutants of Bradyrhizobium japonicum USDA122 for the type III protein secretion system (T3SS) failed to secrete typical effector proteins and gained the ability to nodulate Rj2 soybean plants (Hardee), which are symbiotically incompatible with wild-type USDA122. This suggests that effectors secreted via the T3SS trigger incompatibility between these two partners.

FOOTNOTES

    • Received 26 October 2012.
    • Accepted 26 November 2012.
    • Accepted manuscript posted online 30 November 2012.
  • Supplemental material for this article may be found at http://dx.doi.org/10.1128/AEM.03297-12.

  • Copyright © 2013, American Society for Microbiology. All Rights Reserved.
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The Type III Secretion System of Bradyrhizobium japonicum USDA122 Mediates Symbiotic Incompatibility with Rj2 Soybean Plants
Takahiro Tsukui, Shima Eda, Takakazu Kaneko, Shusei Sato, Shin Okazaki, Kaori Kakizaki-Chiba, Manabu Itakura, Hisayuki Mitsui, Akifumi Yamashita, Kimihiro Terasawa, Kiwamu Minamisawa
Applied and Environmental Microbiology Jan 2013, 79 (3) 1048-1051; DOI: 10.1128/AEM.03297-12

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The Type III Secretion System of Bradyrhizobium japonicum USDA122 Mediates Symbiotic Incompatibility with Rj2 Soybean Plants
Takahiro Tsukui, Shima Eda, Takakazu Kaneko, Shusei Sato, Shin Okazaki, Kaori Kakizaki-Chiba, Manabu Itakura, Hisayuki Mitsui, Akifumi Yamashita, Kimihiro Terasawa, Kiwamu Minamisawa
Applied and Environmental Microbiology Jan 2013, 79 (3) 1048-1051; DOI: 10.1128/AEM.03297-12
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