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Biotechnology

Combined Use of Bacteriophage K and a Novel Bacteriophage To Reduce Staphylococcus aureus Biofilm Formation

D. R. Alves, A. Gaudion, J. E. Bean, P. Perez Esteban, T. C. Arnot, D. R. Harper, W. Kot, L. H. Hansen, M. C. Enright, A. Tobias A. Jenkins
J. L. Schottel, Editor
D. R. Alves
aDepartment of Chemistry, University of Bath, Claverton Down, Bath, United Kingdom
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A. Gaudion
aDepartment of Chemistry, University of Bath, Claverton Down, Bath, United Kingdom
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J. E. Bean
aDepartment of Chemistry, University of Bath, Claverton Down, Bath, United Kingdom
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P. Perez Esteban
bDepartment of Chemical Engineering, University of Bath, Claverton Down, Bath, United Kingdom
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T. C. Arnot
bDepartment of Chemical Engineering, University of Bath, Claverton Down, Bath, United Kingdom
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D. R. Harper
cAmpliPhi Biosciences Corp., Colworth Science Park, Sharnbrook, Bedfordshire, United Kingdom
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W. Kot
dSection of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark
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L. H. Hansen
dSection of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark
eDepartment of Environmental Science, Aarhus Universitet, Roskilde, Denmark
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M. C. Enright
aDepartment of Chemistry, University of Bath, Claverton Down, Bath, United Kingdom
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A. Tobias A. Jenkins
aDepartment of Chemistry, University of Bath, Claverton Down, Bath, United Kingdom
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J. L. Schottel
Roles: Editor
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DOI: 10.1128/AEM.01789-14
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ABSTRACT

Biofilms are major causes of impairment of wound healing and patient morbidity. One of the most common and aggressive wound pathogens is Staphylococcus aureus, displaying a large repertoire of virulence factors and commonly reduced susceptibility to antibiotics, such as the spread of methicillin-resistant S. aureus (MRSA). Bacteriophages are obligate parasites of bacteria. They multiply intracellularly and lyse their bacterial host, releasing their progeny. We isolated a novel phage, DRA88, which has a broad host range among S. aureus bacteria. Morphologically, the phage belongs to the Myoviridae family and comprises a large double-stranded DNA (dsDNA) genome of 141,907 bp. DRA88 was mixed with phage K to produce a high-titer mixture that showed strong lytic activity against a wide range of S. aureus isolates, including representatives of the major international MRSA clones and coagulase-negative Staphylococcus. Its efficacy was assessed both in planktonic cultures and when treating established biofilms produced by three different biofilm-producing S. aureus isolates. A significant reduction of biofilm biomass over 48 h of treatment was recorded in all cases. The phage mixture may form the basis of an effective treatment for infections caused by S. aureus biofilms.

FOOTNOTES

    • Received 30 May 2014.
    • Accepted 19 August 2014.
    • Accepted manuscript posted online 22 August 2014.
  • Supplemental material for this article may be found at http://dx.doi.org/10.1128/AEM.01789-14.

  • Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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Combined Use of Bacteriophage K and a Novel Bacteriophage To Reduce Staphylococcus aureus Biofilm Formation
D. R. Alves, A. Gaudion, J. E. Bean, P. Perez Esteban, T. C. Arnot, D. R. Harper, W. Kot, L. H. Hansen, M. C. Enright, A. Tobias A. Jenkins
Applied and Environmental Microbiology Oct 2014, 80 (21) 6694-6703; DOI: 10.1128/AEM.01789-14

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Combined Use of Bacteriophage K and a Novel Bacteriophage To Reduce Staphylococcus aureus Biofilm Formation
D. R. Alves, A. Gaudion, J. E. Bean, P. Perez Esteban, T. C. Arnot, D. R. Harper, W. Kot, L. H. Hansen, M. C. Enright, A. Tobias A. Jenkins
Applied and Environmental Microbiology Oct 2014, 80 (21) 6694-6703; DOI: 10.1128/AEM.01789-14
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