Article Figures & Data
Figures
- FIG 1
Sucrose enhances S. mutans attachment and biofilm formation. The levels of S. mutans attachment (A) and biofilm formation (B) on glass are significantly enhanced at all time points when the bacteria are grown in BHI containing 1% sucrose (Medium+Sucrose) compared to the levels achieved in BHI containing 1% glucose (Medium+Glucose). S. mutans attachment (C) and biofilm formation (D) on hydroxyapatite are similarly increased in the presence of sucrose, illustrating that the effect of sucrose on S. mutans physiology is not surface specific. Fluorescence microscopy images verify the findings of the CFU count experiments by showing an increase in S. mutans attachment (E) and biofilm formation (F) on glass in the presence of sucrose. *, statistically significant difference determined by Student's t test (P < 0.02). Error bars represent SDs. Scale bars, 20 μm.
- FIG 2
Supplemental sugar alters S. mutans growth. A growth curve of S. mutans in BHI with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans' growth rate changes based on the specific sugar present in the growth medium. Error bars represent SDs.
- FIG 3
Salivary mucins reduce S. mutans attachment and biofilm formation. The addition of 0.3% mucins to the control medium, BHI containing 1% sucrose (SMedium), significantly reduces the levels of S. mutans attachment and biofilm formation on glass (A, B) and hydroxyapatite (C, D) compared to the levels obtained with the control consisting of BHI with 1% sucrose. Similarly, the addition of 0.3% methylcellulose to BHI with 1% sucrose reduces S. mutans attachment and biofilm formation; however, the effect is not significant for the majority of time points studied. Fluorescence microscopy was used to visually assess S. mutans attachment (E) and biofilm formation (F) on glass when the bacteria are grown in BHI with 1% sucrose and 0.3% mucins, BHI with 1% sucrose and 0.3% methylcellulose (Methyl.), and BHI with 1% sucrose. *, statistically significant difference from BHI with 1% sucrose determined by Student's t test (P < 0.02). Error bars represent SDs. Scale bars, 20 μm.
- FIG 4
S. mutans growth is unaffected by the presence of salivary mucins. A growth curve of S. mutans in BHI with 1% sucrose (SMedium), BHI with 1% sucrose and 0.3% mucins, or BHI with 1% sucrose and 0.3% methylcellulose indicates that the presence of mucins and methylcellulose does not alter the growth of S. mutans. Error bars represent SDs.
- FIG 5
S. mutans survival is unaffected by salivary mucins. The graph represents the total number of viable S. mutans cells per well in the supernatant and biofilm in BHI with 1% sucrose (SMedium), BHI with 1% sucrose and 0.3% mucins, or BHI with 1% sucrose and 0.3% methylcellulose. Salivary mucins and methylcellulose show no bactericidal effects at time points up to 24 h. Error bars represent SDs.
- FIG 6
Summary of conclusions. S. mutans utilizes sucrose to form sticky extracellular polysaccharides that facilitate attachment to the tooth surface and subsequent biofilm formation. (A) In the biofilm, bacterial metabolism of sucrose causes a decrease in the local pH, leading to demineralization of the tooth structure. (B) The presence of mucins in sucrose-supplemented growth medium decreases S. mutans attachment and biofilm formation on the tooth surface by maintaining S. mutans in the planktonic state.
