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Genetics and Molecular Biology | Spotlight

Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484

Paula M. O'Connor, Eileen F. O'Shea, Caitriona M. Guinane, Orla O'Sullivan, Paul D. Cotter, R. Paul Ross, Colin Hill
C. A. Elkins, Editor
Paula M. O'Connor
Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, IrelandAlimentary Pharmabiotic Centre, Cork, Ireland
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Eileen F. O'Shea
Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, IrelandAlimentary Pharmabiotic Centre, Cork, Ireland
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Caitriona M. Guinane
Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland
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Orla O'Sullivan
Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland
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Paul D. Cotter
Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, IrelandAlimentary Pharmabiotic Centre, Cork, Ireland
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R. Paul Ross
College of Science, Engineering and Food Science, University College Cork, Cork, IrelandAlimentary Pharmabiotic Centre, Cork, Ireland
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Colin Hill
School of Microbiology, University College Cork, Cork, IrelandAlimentary Pharmabiotic Centre, Cork, Ireland
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C. A. Elkins
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DOI: 10.1128/AEM.00212-15
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ABSTRACT

Accumulating evidence suggests that bacteriocin production represents a probiotic trait for intestinal strains to promote dominance, fight infection, and even signal the immune system. In this respect, in a previous study, we isolated from the porcine intestine a strain of Streptococcus hyointestinalis DPC6484 that displays antimicrobial activity against a wide range of Gram-positive bacteria and produces a bacteriocin with a mass of 3,453 Da. Interestingly, the strain was also found to be immune to a nisin-producing strain. Genome sequencing revealed the genetic determinants responsible for a novel version of nisin, designated nisin H, consisting of the nshABTCPRKGEF genes, with transposases encoded between nshP and nshR and between nshK and nshG. A similar gene cluster is also found in S. hyointestinalis LMG14581. Notably, the cluster lacks an equivalent of the nisin immunity gene, nisI. Nisin H is proposed to have the same structure as the prototypical nisin A but differs at 5 amino acid positions—Ile1Phe (i.e., at position 1, nisin A has Ile while nisin H has Phe), Leu6Met, Gly18Dhb (threonine dehydrated to dehydrobutyrine), Met21Tyr, and His31Lys—-and appears to represent an intermediate between the lactococcal nisin A and the streptococcal nisin U variant of nisin. Purified nisin H inhibits a wide range of Gram-positive bacteria, including staphylococci, streptococci, Listeria spp., bacilli, and enterococci. It represents the first example of a natural nisin variant produced by an intestinal isolate of streptococcal origin.

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Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484
Paula M. O'Connor, Eileen F. O'Shea, Caitriona M. Guinane, Orla O'Sullivan, Paul D. Cotter, R. Paul Ross, Colin Hill
Appl. Environ. Microbiol. May 2015, 81 (12) 3953-3960; DOI: 10.1128/AEM.00212-15

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Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484
Paula M. O'Connor, Eileen F. O'Shea, Caitriona M. Guinane, Orla O'Sullivan, Paul D. Cotter, R. Paul Ross, Colin Hill
Appl. Environ. Microbiol. May 2015, 81 (12) 3953-3960; DOI: 10.1128/AEM.00212-15
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