Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AEM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Applied and Environmental Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AEM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Geomicrobiology

Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients

Weimin Sun, Tangfu Xiao, Min Sun, Yiran Dong, Zengping Ning, Enzong Xiao, Song Tang, Jiwei Li
G. Voordouw, Editor
Weimin Sun
aState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China
bDepartment of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tangfu Xiao
aState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Min Sun
aState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yiran Dong
cDepartment of Geology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zengping Ning
aState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Enzong Xiao
aState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Song Tang
dSchool of Environment and Sustainability, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiwei Li
eSanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G. Voordouw
Roles: Editor
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/AEM.00935-15
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Additional Files
  • FIG 1
    • Open in new tab
    • Download powerpoint
    FIG 1

    Location of the Aha watershed in China and the locations of 11 sampling sites in the Aha watershed. Please note that water flows from SD1 to SD13. The map was created using CorelDraw.

  • FIG 2
    • Open in new tab
    • Download powerpoint
    FIG 2

    Taxonomic classification of bacterial reads retrieved from different watershed samples at phylum level using the RDP classifier. “Rare” indicates phyla with relative abundances of less than 0.5%.

  • FIG 3
    • Open in new tab
    • Download powerpoint
    FIG 3

    Heat map analysis of the distribution of dominant phylotypes in the 11 samples. The double hierarchical dendrogram shows the microbial distribution of the 11 samples. The relative percentages for the microbial genera are depicted by the color intensity; the color key is at the bottom.

  • FIG 4
    • Open in new tab
    • Download powerpoint
    FIG 4

    UPGMA tree showing clusters of microbial communities based on weighted UniFrac with 100% support at all nodes.

  • FIG 5
    • Open in new tab
    • Download powerpoint
    FIG 5

    Ordination diagrams from canonical correspondence analysis (CCA) of bacterial abundances and geochemical parameters. Response variables of bacterial abundances are indicated by triangles. Arrows indicate the direction and magnitude of geochemical parameters associated with bacterial community structures. Each sample is represented by colored circles according to sampling zones. Abbreviations: Temp, temperature; Fetot, total Fe; TC, total carbon in the sediments; TOC, total organic carbon in the sediments. Bacterial abbreviations: Halo, Halomonas; Shewa, Shewanella; Mon, Desulfomonile; Geo, Geobacter; Syn, Syntrophobacter; Acidi, Aciditerrimonas; Alicyclo, Alicyclobacillus; Desulfos, Desulfosporosinus.

  • FIG 6
    • Open in new tab
    • Download powerpoint
    FIG 6

    Geomicrobiological model of the Aha watershed profile. The model shows the roles of the major S- and Fe-related bacteria identified in the current study. The color of the arrows indicates the extent of the physiochemical parameters or geochemical process. A transition from saturated to transparent colors in a color ribbon indicates a decrease in the corresponding environmental factor from zone 1 to zone 4 and vice versa. Abbreviations: FeOB, Fe(II)-oxidizing bacteria; FeRB, Fe(III)-reducing bacteria; SOB, sulfur-oxidizing bacteria; SRB, sulfate-reducing bacteria; Fetot: total Fe; PPT, precipitation.

Tables

  • Figures
  • Additional Files
  • TABLE 1

    Physiochemical parameters in the water samples taken from the Aha watersheda

    SamplepHTemp (°C)Eh (mV)TDS (g/liter)Conductivity (mS/cm)Salinity (‰)Fetot (mg/liter)Fe(II) (mg/liter)SO42− (mg/liter)
    B12.8120.9375.13.355.83.441,3041,2586,646
    B22.8125.4419.23.196.193.271,2739716,838
    SD18.115.520.80.2510.4220.250.015ND112
    SD26.815.620.30.3060.5160.310.014ND219
    SD38.1215.735.30.3470.5810.350.035ND254
    SD48.1115.9530.4670.780.470.035ND412
    SD57.5918.9600.2690.4870.270.015ND166
    SD97.5718.873.10.260.470.260.015ND151
    SD137.4417.5−183.60.320.5590.320.009ND157
    • ↵a Abbreviations: TDS, total dissolved solids; Fetot, total iron; Fe(II), ferrous; ND, not detected.

  • TABLE 2

    The pHs and concentrations of total nitrogen, total carbon, organic carbon, total hydrogen, and total and soluble sulfur in 11 sediment samplesa

    Geographical zoneSamplepHConcn (g/kg) of:
    TNTCTOCTSSSH
    Zone 1B12.150.19741.22140.5320.58210.5550.2447
    B22.020.02160.14260.1360.58750.5630.186
    Zone 2SD17.760.0220.92810.2490.06060.0510.0736
    SD27.390.03270.4650.3490.04170.0290.1379
    SD37.840.02421.04770.8550.12190.0810.1188
    SD47.660.02671.0630.770.11210.0710.1236
    Zone 3SD57.40.02720.80250.2620.08050.0320.0949
    SD66.660.02180.80030.6330.18790.1510.1388
    SD76.480.02380.86870.6750.17010.1330.1442
    SD97.250.02980.46970.2710.10020.0830.1022
    Zone 4SD136.860.0530.77690.5650.48780.4230.1453
    • ↵a Abbreviations: TN, total nitrogen; TC, total carbon; TOC, total organic carbon; TS, total sulfur; SS, soluble sulfur; H, total hydrogen.

Additional Files

  • Figures
  • Tables
  • Supplemental material

    • Supplemental file 1 -

      Major elemental concentrations in the AMD sediment samples from Aha watershed (Table S1); OTU numbers and Chao1 and Shannon indices for all samples (Table S2); microbial community composition, shown as relative abundances in the AMD watershed at the phylum level (Table S3); Fe- and S-related bacteria detected in the present study and their relative abundances in the Aha watershed (Table S4); the major components of zone 1 (Fig. S1).

      PDF, 630K

PreviousNext
Back to top
Download PDF
Citation Tools
Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients
Weimin Sun, Tangfu Xiao, Min Sun, Yiran Dong, Zengping Ning, Enzong Xiao, Song Tang, Jiwei Li
Applied and Environmental Microbiology Jul 2015, 81 (15) 4874-4884; DOI: 10.1128/AEM.00935-15

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Applied and Environmental Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients
(Your Name) has forwarded a page to you from Applied and Environmental Microbiology
(Your Name) thought you would be interested in this article in Applied and Environmental Microbiology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients
Weimin Sun, Tangfu Xiao, Min Sun, Yiran Dong, Zengping Ning, Enzong Xiao, Song Tang, Jiwei Li
Applied and Environmental Microbiology Jul 2015, 81 (15) 4874-4884; DOI: 10.1128/AEM.00935-15
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About AEM
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #AppEnvMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

 

Print ISSN: 0099-2240; Online ISSN: 1098-5336