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Applied and Environmental Microbiology
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Environmental Microbiology

Transcriptomics-Aided Dissection of the Intracellular and Extracellular Roles of Microcystin in Microcystis aeruginosa PCC 7806

A. Katharina Makower, J. Merijn Schuurmans, Detlef Groth, Yvonne Zilliges, Hans C. P. Matthijs, Elke Dittmann
R. M. Kelly, Editor
A. Katharina Makower
aUniversity of Potsdam, Institute for Biochemistry and Biology, Department of Microbiology, Potsdam-Golm, Germany
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J. Merijn Schuurmans
bUniversity of Amsterdam, Institute for Biodiversity and Ecosystem Dynamics, Department of Aquatic Microbiology, Amsterdam, The Netherlands
cNetherlands Institute for Ecology, NIOO-KNAW, Department of Aquatic Ecology, Wageningen, The Netherlands
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Detlef Groth
dUniversity of Potsdam, Institute for Biochemistry and Biology, Department of Bioinformatics, Potsdam-Golm, Germany
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Yvonne Zilliges
eFree University of Berlin, Institute of Experimental Physics, Department of Biophysics and Photosynthesis, Berlin, Germany
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Hans C. P. Matthijs
bUniversity of Amsterdam, Institute for Biodiversity and Ecosystem Dynamics, Department of Aquatic Microbiology, Amsterdam, The Netherlands
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Elke Dittmann
aUniversity of Potsdam, Institute for Biochemistry and Biology, Department of Microbiology, Potsdam-Golm, Germany
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R. M. Kelly
Roles: Editor
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DOI: 10.1128/AEM.02601-14
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ABSTRACT

Recent studies have provided evidence for both intracellular and extracellular roles of the potent hepatotoxin microcystin (MC) in the bloom-forming cyanobacterium Microcystis. Here, we surveyed transcriptomes of the wild-type strain M. aeruginosa PCC 7806 and the microcystin-deficient ΔmcyB mutant under low light conditions with and without the addition of external MC of the LR variant (MC-LR). Transcriptomic data acquired by microarray and quantitative PCR revealed substantial differences in the relative expression of genes of the central intermediary metabolism, photosynthesis, and energy metabolism. In particular, the data provide evidence for a lower photosystem I (PSI)-to-photosystem II (PSII) ratio and a more pronounced carbon limitation in the microcystin-deficient mutant. Interestingly, only 6% of the transcriptional differences could be complemented by external microcystin-LR addition. This MC signaling effect was seen exclusively for genes of the secondary metabolism category. The orphan polyketide synthase gene cluster IPF38-51 was specifically downregulated in response to external MC-LR under low light. Our data suggest a hierarchical and light-dependent cross talk of secondary metabolites and support both an intracellular and an extracellular role of MC in Microcystis.

FOOTNOTES

    • Received 8 August 2014.
    • Accepted 18 October 2014.
    • Accepted manuscript posted online 7 November 2014.
  • Supplemental material for this article may be found at http://dx.doi.org/10.1128/AEM.02601-14.

  • Copyright © 2015, American Society for Microbiology. All Rights Reserved.
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Transcriptomics-Aided Dissection of the Intracellular and Extracellular Roles of Microcystin in Microcystis aeruginosa PCC 7806
A. Katharina Makower, J. Merijn Schuurmans, Detlef Groth, Yvonne Zilliges, Hans C. P. Matthijs, Elke Dittmann
Applied and Environmental Microbiology Jan 2015, 81 (2) 544-554; DOI: 10.1128/AEM.02601-14

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Transcriptomics-Aided Dissection of the Intracellular and Extracellular Roles of Microcystin in Microcystis aeruginosa PCC 7806
A. Katharina Makower, J. Merijn Schuurmans, Detlef Groth, Yvonne Zilliges, Hans C. P. Matthijs, Elke Dittmann
Applied and Environmental Microbiology Jan 2015, 81 (2) 544-554; DOI: 10.1128/AEM.02601-14
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