Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • Log out
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AEM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • Log out
  • My Cart

Search

  • Advanced search
Applied and Environmental Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AEM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Physiology | Spotlight

A New Class of Tungsten-Containing Oxidoreductase in Caldicellulosiruptor, a Genus of Plant Biomass-Degrading Thermophilic Bacteria

Israel M. Scott, Gabe M. Rubinstein, Gina L. Lipscomb, Mirko Basen, Gerrit J. Schut, Amanda M. Rhaesa, W. Andrew Lancaster, Farris L. Poole, II, Robert M. Kelly, Michael W. W. Adams
M. J. Pettinari, Editor
Israel M. Scott
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gabe M. Rubinstein
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gina L. Lipscomb
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mirko Basen
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gerrit J. Schut
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amanda M. Rhaesa
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
W. Andrew Lancaster
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Farris L. Poole
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert M. Kelly
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael W. W. Adams
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. J. Pettinari
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/AEM.01634-15
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

ABSTRACT

Caldicellulosiruptor bescii grows optimally at 78°C and is able to decompose high concentrations of lignocellulosic plant biomass without the need for thermochemical pretreatment. C. bescii ferments both C5 and C6 sugars primarily to hydrogen gas, lactate, acetate, and CO2 and is of particular interest for metabolic engineering applications given the recent availability of a genetic system. Developing optimal strains for technological use requires a detailed understanding of primary metabolism, particularly when the goal is to divert all available reductant (electrons) toward highly reduced products such as biofuels. During an analysis of the C. bescii genome sequence for oxidoreductase-type enzymes, evidence was uncovered to suggest that the primary redox metabolism of C. bescii has a completely uncharacterized aspect involving tungsten, a rarely used element in biology. An active tungsten utilization pathway in C. bescii was demonstrated by the heterologous production of a tungsten-requiring, aldehyde-oxidizing enzyme (AOR) from the hyperthermophilic archaeon Pyrococcus furiosus. Furthermore, C. bescii also contains a tungsten-based AOR-type enzyme, here termed XOR, which is phylogenetically unique, representing a completely new member of the AOR tungstoenzyme family. Moreover, in C. bescii, XOR represents ca. 2% of the cytoplasmic protein. XOR is proposed to play a key, but as yet undetermined, role in the primary redox metabolism of this cellulolytic microorganism.

INTRODUCTION

Thermophilic bacteria of the genus Caldicellulosiruptor are currently under intense investigation due to their ability to decompose lignocellulosic plant biomass anaerobically at high temperature, thereby potentially mitigating costly thermochemical pretreatment steps (1, 2). One of these species, Caldicellulosiruptor bescii, has an optimal growth temperature of 78°C and is the most thermophilic cellulose degrader known to date. It is able to ferment high concentrations of cellulosic feedstock primarily to hydrogen gas, lactate, acetate, and CO2 (3, 4). Species from this genus can degrade cellulose (and also xylan), using novel multidomain glycosyl hydrolases, representing a new paradigm in cellulose conversion by anaerobic thermophiles (2). Moreover, the recent development of a genetic system for C. bescii creates potential for using this and related species for consolidated biomass processing in the production of liquid fuels (5).

Developing metabolic engineering strategies for any microorganism obviously requires an in-depth understanding of their primary metabolism. Evidence that C. bescii may have a completely uncharacterized aspect to its primary redox metabolism came from an analysis of its genome sequence for molybdoenzymes (6). These are present in virtually all forms of life, serving diverse roles in primary metabolism of carbon, nitrogen and sulfur (7). As expected, we found that the C. bescii genome contains genes necessary for the synthesis of the pyranopterin cofactor that coordinates molybdenum (Mo) in such enzymes (7). Accordingly, the genome also contains a gene (Athe_1215) encoding a member of the dimethyl sulfoxide reductase (DMSOR) family, the most diverse of the three classes of molybdoenzyme (that also includes the xanthine oxidase and sulfite oxidase families [7, 8]). Unexpectedly, however, C. bescii contains the tupABC operon, which encodes an ABC transporter that is highly specific for the uptake of the analogous metal tungsten (9), instead of the typical modABC genes that encode the uptake of molybdenum.

That C. bescii might utilize tungsten was very surprising. Although molybdenum-containing enzymes are ubiquitous in biology, microorganisms that require tungsten are extremely limited (10). Indeed, tungsten and molybdenum have such similar chemical and physical properties that almost all microorganisms cannot distinguish between them and often times incorporate tungsten into their molybdoenzymes. This typically renders them nonfunctional (11), although tungsten can be incorporated into some members of the DMSOR family (formate dehydrogenase, formyl methanofuran dehydrogenase, and acetylene hydratase) to yield active enzyme (12). Only a very few microorganisms are known to absolutely require tungsten for growth, and they incorporate it into the so-called true family of tungstoenzymes represented by aldehyde ferredoxin oxidoreductase (AOR) (12, 13). The AOR family of tungstoenzymes is unrelated phylogenetically to the three families of molybdoenzymes (8, 14), although like the molybdenum in molybdoenzymes, the tungsten in the AOR family is coordinated by a pyranopterin cofactor (14, 15).

The best-characterized microorganisms that contain the AOR family of tungstoenzymes are members of the hyperthermophilic archaea, represented by Pyrococcus furiosus, which grows optimally at 100°C. Such organisms have high selectivity for the two metals. For example, P. furiosus does not incorporate significant amounts of molybdenum into its AOR, even when the organism is grown in a 40-fold excess of molybdenum over tungsten (16). P. furious grows by fermenting sugars (but not cellulose) and peptides and contains five members of the AOR family (abbreviated AOR [17], GAPOR [18], FOR [19], WOR4 [20], and WOR5 [21]), all of which oxidize aldehydes of various types. The prototypical AOR has a broad substrate specificity and is thought to be involved in peptide catabolism wherein it oxidizes amino acid-derived aldehydes (10).

It was therefore surprising to find that the C. bescii genome not only encodes a tungstate transporter but also contains a gene (Athe_0821) annotated as a member of the AOR family. This suggests that C. bescii possesses a previously unknown ability to utilize tungsten. Here, we show that this is indeed the case by demonstrating heterologous production of active, tungsten-containing P. furiosus AOR in C. bescii. Moreover, the “AOR” of C. bescii is phylogenetically unique and represents a sixth distinct member of the AOR family. This new type of tungstoenzyme is proposed to play a key role in the primary redox metabolism of this cellulolytic microorganism.

MATERIALS AND METHODS

Strains and growth conditions.C. bescii strains used or constructed in the present study are listed in Table 1. Low-osmolarity defined (LOD) medium (22) was prepared from filter sterilized stock solutions. The 50× base salts solution contained 16.5 g of MgCl2, 16.5 g of KCl, 12.5 g of NH4Cl, 7 g of CaCl2·2H2O, and 0.68 g of KH2PO4 per liter. Trace element solution SL-10 is prepared as described previously (23), and the 200× vitamin solution contained the following vitamins (in milligrams) per liter: biotin, 4; folic acid, 4; pyridoxine-HCl, 20; riboflavin, 10; thiamine-HCl, 10; nicotinic acid 10; pantothenic acid, 10; vitamin B12, 0.2; p-aminobenzoic acid, 10; and lipoic acid, 10. Unless otherwise indicated, C. bescii was routinely cultured under strict anaerobic conditions at 75°C with shaking at 200 rpm in LOD medium (22), with the exception that maltose was replaced with cellobiose and sodium molybdate and sodium tungstate were added at final concentrations of 1 μM. Pyrococcus furiosus strain COM1 (24) was cultured under strict anaerobic conditions at 90°C in static bottles in artificial seawater medium containing per liter: 5 g of maltose, 1× base salts (23), 1× trace minerals (23), 10 μM sodium tungstate, 0.25 μg of resazurin, 2 g of yeast extract, 0.5 g of cysteine, 0.5 g sodium sulfide, 1 g of sodium bicarbonate, 1 mM potassium phosphate buffer (pH 6.8), and 20 μM uracil.

View this table:
  • View inline
  • View popup
TABLE 1

Strains used and constructed in this study

RNA isolation and quantitative reverse transcription-PCR (RT-PCR).C. bescii was grown in 100-ml sealed serum bottles with 50 ml of LOD medium containing cellobiose (5 g liter−1) at 75°C until mid-exponential phase (optical density at 680 nm of 0.06 to 0.08). Cultures were cooled to 4°C, cells were harvested by centrifugation, and cell pellets were stored at −80°C. For total RNA isolation, frozen cell pellets were suspended in 300 μl of lysis buffer (4 M guanidine thiocyanate, 0.83% N-lauryl sarcosine [pH 5]), followed by the addition of 300 μl of acid-equilibrated phenol-chloroform (5:1; pH 4.3 to 4.7; Sigma). After vortexing the tubes to form an even suspension, the suspended cells were subjected to three 10-s intervals of sonication (amplitude 40; Qsonica Q55), interspaced by at least 30 s. The resulting cell lysate was mixed with 600 μl of 100% ethanol, and total RNA was isolated using a Direct-Zol RNA MiniPrep kit (Zymo Research), according to the manufacturer's protocol, with the exception that genomic DNA was digested in solution as opposed to on the column using Turbo DNase (Ambion). RNA was quantified with a Nano-Drop 2000c spectrometer (Thermo Scientific). Synthesis of cDNA was performed with 1 μg of purified RNA using the Affinity Script QPCR cDNA synthesis kit (Agilent). A Brilliant II SYBR green QPCR master mix (Agilent) was used for quantitative reverse transcription-PCR (RT-PCR) experiments with primers designed to amplify a ∼200-base product within the target genes: Athe_0821 (xor) and PF0346 (aor). For comparison to the Athe_1406 gene (the GAPDH gene) which is expressed at high levels during growth on Avicel, cellobiose, glucose, xylose, and xylan using RNA-seq (data not shown). The primers used in the present study are presented in Table 2.

View this table:
  • View inline
  • View popup
TABLE 2

Primers used in this study

Phylogenetic analysis.BLAST searches of the amino acid sequences of XOR and the unknown dehydrogenase (UDH) were performed against the NCBI database using the default settings. The top 2,000 hits for XOR and the top 10,000 hits for UDH were used to construct phylogenetic trees for each on the basis of neighborhood joining and Jukes-Cantor methods, with 100 bootstrap replicates done for each tree using CLC Main Workbench 6 (CLC Bio).

Plasmid construction.The C. bescii replicating shuttle vector pDCW89 (25) was modified via Gibson Assembly (New England BioLabs) to include a His tag and a multiple cloning site from the commercial vector pET24a (Novagen), generating pIMS89. The P. furiosus aor gene (PF0346) and the C. bescii S-layer protein promoter region (200 bp starting immediately upstream of the start of Athe_2303) were amplified from genomic DNA, spliced together using overlap PCR and cloned into pIMS89 to create pIMSAOR (see Fig. S1 in the supplemental material).

Strain construction.C. bescii strain JWCB018 cells were rendered competent as previously described (26) and transformed using 0.5 μg of purified plasmid pIMSAOR via electroporation by a single electric pulse (2.0 kV, 25 μF, and 200 Ω) in a 1-mm cuvette using a Gene Pulser (Bio-Rad). Transformants were allowed to recover at 75°C in 20 ml of LOC medium (22) for 1 to 2 h, after which cells from 1 ml of the recovery culture were harvested and transferred to LOD medium lacking uracil. These selective outgrowth cultures were incubated 18 to 72 h, and those with appreciable growth were colony purified on solid LOD medium lacking uracil. The strain was verified to contain the pIMSAOR plasmid by PCR screening, as well as backtransforming isolated plasmid into E. coli. The purified strain was designated MACB1002 (Table 1).

Preparation and fractionation of cell extracts for metal analysis.C. bescii strains JWCB018 and MACB1002 were each cultured in 4 liters of LOD medium and harvested in the late-exponential phase by centrifugation at 6,000 × g for 10 min (Beckman Avanti J-30I JLA 10.500 rotor) to yield 3.8 and 3.1 g of cells (wet weight), respectively. Cell pellets were flash frozen in liquid nitrogen and stored at −80°C. Strict anaerobic conditions were maintained for all successive steps. Cell pellets were thawed and suspended in 25 mM Tris buffer (pH 8.0) containing 1 mg ml−1 lysozyme (Sigma-Aldrich) in a ratio of 3 ml per g of cells. The suspended cells were incubated at room temperature for 15 min followed by three 10-s intervals of sonication (amplitude 40; Qsonica Q55) interspaced by at least 30 s. Cell lysates were clarified by ultracentrifugation at 100,000 × g for 1 h (Beckman L90K ultracentrifuge 70.1Ti rotor). Clarified cell lysates from each cell batch were directly loaded onto a 1 ml Hi-trap QHP column (GE Healthcare) equilibrated anaerobically with 25 mM Tris buffer (pH 8.0), using an ÄKTA purifier system (GE Healthcare). Protein was eluted using a linear gradient of 0 to 1 M NaCl in 25 mM Tris buffer (pH 8.0), and fractions were collected anaerobically. Metal concentrations were measured in the cytoplasmic extract and in column fractions using quadrupole-based inductively coupled plasma mass spectroscopy (ICP-MS) as described previously (27). Proteins were identified in the chromatography fractions by liquid chromatography-tandem mass spectrometry (Proteomics and Mass Spectrometry Facility, University of Georgia).

Purification of heterologously expressed AOR from C. bescii.All purification steps were performed under strictly anaerobic conditions. Frozen cell pellets (3.5 g) of C. bescii strain MACB1002 were lysed and a cell-free supernatant (13 ml containing 31.0 mg of protein ml−1) was obtained as described above. This was applied to a 1-ml HisTrap excel column (GE Healthcare) equilibrated in buffer A (50 mM phosphate buffer [pH 7.2], 300 mM NaCl). The column was washed with five volumes of buffer A, and AOR was eluted with a gradient of 0 to 500 mM imidazole in buffer B (buffer A containing 500 mM imidazole) over 20 column volumes. Fractions were collected in 2-ml volumes in sealed serum bottles made anaerobic by degassing with argon. Fractions containing AOR activity were analyzed for purity using denaturing SDS-PAGE gradient electrophoresis (4 to 12% Bis-Tris gels; Novex) and stained with Imperial protein stain (Novex). Purified protein was analyzed for metal content via ICP-MS as described previously (27).

Enzyme assays.The aldehyde oxidizing activity of cell extracts of C. bescii strains JWCB018 and MACB1002 and P. furiosus COM1 were determined by measuring the reduction of benzyl viologen (1 mM) in 50 mM EPPS buffer (pH 8.0) at 75°C in rubber-stoppered cuvettes under anaerobic conditions using various aldehydes (1 mM) as the substrates. To remove trace amounts of O2, sodium dithionite was added to the assay mixture to give an A600 of ∼0.2. The extract was added and, after a 1-min incubation period, the reaction was initiated by addition of the aldehyde. An extinction coefficient of 7.4 mM−1cm−1 was used for reduced benzyl viologen (28). Specific enzyme activities are expressed as units per mg of protein, where one unit represents 1 μmol of aldehyde oxidized per min.

RESULTS

Analysis of the tungsten pyranopterin biosynthetic gene cluster in C. bescii.An analysis of the C. bescii genome revealed that it contains all but one of the genes necessary for the synthesis of the pyranopterin cofactor from GTP (29). These are arranged in a gene cluster (Athe_0822 to Athe_08031) that includes moaABCD, moeA1, moeA2, and moeB (Fig. 1A). Absent from the genome of C. bescii is a gene encoding MoaE. This is very unusual, but not without precedent in microorganisms that utilize tungsten (30). Another unusual feature is the presence of two genes encoding moeA homologs in the C. bescii genome. It has been hypothesized that MoeA functions in metal selectivity between tungsten and molybdenum, but a mechanism for this selectivity has yet to be elucidated (31). As shown in Fig. 1A, instead of the expected modABC genes, which encode a molybdate transporter, the tupABC genes are found, and these encode a transporter specific for tungstate. The relative expression levels of the pyranopterin biosynthesis and tungstate-related genes in wild-type C. bescii grown on cellobiose in the presence of 1 μM tungstate and 1 μM molybdate are shown in Fig. 1B. All of the genes are expressed at least an order of magnitude lower than that of the gene encoding the glycolytic enzyme, glyeraldehyde-3-phosphate dehydrogenase (GAPDH), with the exception of moeB, which is encoded within a separate upstream gene cluster. This gene is at least 20-fold higher in expression than the GAPDH gene, and perhaps higher expression is needed because of the role of MoeB in recycling MoaD (8, 32).

FIG 1
  • Open in new tab
  • Download powerpoint
FIG 1

(A) C. bescii gene cluster (Athe_0820 to Athe_0831) encoding the proteins necessary for pyranopterin biosynthesis (green) and tungstate transport (blue), as well as a ferredoxin (orange) and XOR (orange). (B) Expression levels of the genes encoding pyranopterin biosynthesis (moeB, moeA1, moeA2, and moaABC), tungstate transport (tupABC), ferredoxin (Fd gene) and XOR (xor) relative to that of the gene encoding the glycolytic enzyme GAPDH, as determined by quantitative RT-PCR. Bars are color-coded according to genes in part A. Error bars represent the standard deviations (SD; n = 3 technical replicates).

Analyses of C. bescii tungsten utilization by heterologous expression of AOR.To determine whether the pyranopterin and tungstate-related genes in C. bescii were fully functional and could support the uptake of tungstate and its incorporation into a known tungstoenzyme, the organism was engineered to heterologously express an affinity-tagged version of AOR from P. furiosus, one of the most well-characterized tungstoenzymes (14). The gene encoding P. furiosus AOR (PF0346) was modified to include an N-terminal polyhistidine tag, and its expression was placed under the control of the promoter of the gene (slp) encoding the S-layer protein of C. bescii. Under standard growth conditions, the slp gene is expressed at a level that is about 10-fold higher than that of the GAPDH gene in wild-type C. bescii (Fig. 2A). The aor expression construct was inserted into a shuttle vector (see Fig. S1 in the supplemental material), and this was transformed into the genetic background strain JWCB018 (33) to create strain MACB1002 (Table 1). Analysis of the AOR gene expression level in strain MACB1002 revealed that it was higher than slp (Fig. 2A). In spite of the extremely high expression level of aor, strain MACB1002 exhibited no obvious growth phenotype when cultured on a cellobiose-containing medium with 1 μM W and 1 μM Mo (Fig. 2B).

FIG 2
  • Open in new tab
  • Download powerpoint
FIG 2

High-level expression of AOR in C. bescii does not affect growth. (A) Quantitative RT-PCR expression levels of C. bescii slp and P. furiosus aor in C. bescii strain MACB1002 relative to the gene encoding the glycolytic enzyme GAPDH. (B) Growth curves of wild-type (green triangles), genetic background strain JWCB018 (red squares), and P. furiosus AOR-expressing strain MACB1002 (blue diamonds) grown on LOD medium supplemented with 1 μM tungsten and 1 μM molybdenum. The error bars represent the SD (n = 3).

AOR is highly expressed in P. furiosus, and in in vitro assays exhibits high activity using acetaldehyde (1.0 mM) as the substrate with the dye benzyl viologen as the electron acceptor (17). The specific activity in a cell extract of P. furiosus was 4.0 ± 0.1 U/mg at 75°C. The cell extract of the parent C. bescii strain contained no detectable AOR activity (<0.01 U/mg), using acetaldehyde as the substrate. However, the cell extract of the MACB1002 strain, harvested at the end of exponential growth, contained 3.5 ± 1 U/mg, showing that the P. furiosus enzyme was produced in C. bescii at a level comparable to that in its native organism.

P. furiosus AOR was purified from a cell extract of C. bescii strain MACB1002 by a single affinity chromatography step, yielding an enzyme that was close to homogeneity by SDS-PAGE analysis (Fig. 3). Although AOR is a homodimeric enzyme, it is known to migrate as two bands corresponding to the denatured monomeric and the undenatured dimeric forms of the enzyme (17). Purification resulted in relatively high recovery of AOR activity (45%; Table 3), and the specific activity of purified AOR with acetaldehyde (49 U/mg) was comparable to that measured with native P. furiosus AOR (17). This enzyme has been shown to be dimeric with each subunit containing one W and four Fe per monomer and an additional iron per monomer that is shared between the two subunits (14). ICP-MS analysis of P. furiosus AOR from C. bescii yielded an iron to tungsten ratio (Fe/W) of 4.25 ± 0.25, which is close to the value of 4.5 for the pure enzyme. Purified AOR contained only trace amounts of molybdenum with a W/Mo ratio of 62:1. These results, therefore, demonstrate that when C. bescii is grown in the presence of 1 μM W and 1 μM Mo, it is highly selective for tungsten, similar to P. furiosus. Moreover, the level of expression of the pyranopterin and tungstate-related genes in C. bescii can generate a high cellular concentration of recombinant P. furiosus AOR that is very active, contains tungsten rather than molybdenum in its active site, and has the characteristics of natively purified AOR.

FIG 3
  • Open in new tab
  • Download powerpoint
FIG 3

SDS-PAGE analysis of P. furiosus AOR purified from C. bescii strain MACB1002 by a single affinity chromatography step. Gel annotations are as follows: S100, cytoplasmic fraction; FT, flowthrough; fractions, nickel-NTA elution fractions 1 to 7; native AOR, AOR purified from P. furiosus. The enzyme exhibits two bands on an SDS gel corresponding to the denatured monomeric and the undenatured dimeric forms, as previously observed (17).

View this table:
  • View inline
  • View popup
TABLE 3

Purification of P. furiosus AOR from C. bescii strain MACB1002

C. bescii contains an AOR homolog.As shown in Fig. 1A, adjacent to the genes encoding pyranopterin biosynthesis and tungstate transport in C. bescii are two genes that are annotated as aldehyde ferredoxin oxidoreductase (Athe_0821) and ferredoxin (Athe_0820). Athe_0821 encodes a protein (586 residues) that shows 31% sequence similarity to P. furiosus AOR (605 residues), and Athe_0820 is predicted to encode a polyferredoxin with the potential to contain four [4Fe-4S] clusters according to its cysteine motifs. This 12-gene cluster (Athe_0820 to Athe_0831) is conserved in the genomes of the eight Caldicellulosiruptor species that have been sequenced to date, and there is high identity among the AOR homologs (94 to 99%: see Table S1 in the supplemental material), suggesting that the AOR homolog has an important role in this group of microorganisms.

In C. bescii, the genes encoding the AOR homolog (Athe_0821) and its associated ferredoxin (Athe_0820) are among the most highly transcribed genes during growth on glucose, cellobiose, cellulose, and switchgrass, and they do not appear to be significantly regulated under any of these growth conditions, according to DNA microarray data (3). As shown in Fig. 1B, quantitative PCR analysis shows that during growth on cellobiose the genes encoding the AOR homolog and the ferredoxin are expressed at levels similar to that of the gene encoding the glycolytic enzyme GAPDH, and these are an order of magnitude higher than those genes encoding pyranopterin biosynthesis and tungstate transport (Fig. 1B). As noted above, the cytoplasmic fraction of C. bescii cells did not contain significant acetaldehyde-oxidizing activity, which is a characteristic of P. furiosus AOR. The other four members of the AOR family of tungstoenzymes, GAPOR, FOR, WOR4, and WOR5, oxidize a range of other aldehydes, including formaldehyde, propionaldehyde, crotonaldehyde, glutaraldehyde, isovaleraldehyde, benzaldehyde, and glyceraldehyde-3-phosphate (17–21). However, the cell extract of the parent C. bescii strain did not oxidize any of these aldehydes at detectable rates (<0.01 U/mg at 75°C). Given its high expression level in C. bescii, we conclude that the AOR homolog encoded in its genome does not directly correspond to any of the five known members of the AOR family. Henceforth, the C. bescii AOR homolog will be referred to as XOR to indicate that its physiological substrate is not known.

In order to provide additional insights into the role of XOR and tungsten in the metabolism of C. bescii, a cell extract was fractionated by anion-exchange chromatography and the fractions were analyzed for tungsten and molybdenum by ICP-MS. As shown in Fig. 4, a large tungsten peak was observed, which overlaid a minor peak of molybdenum representing ca. 5% of the tungsten. Analysis of the peak tungsten-containing fractions by MS/MS revealed that XOR was a major protein, as indicated by almost complete coverage of the protein by the peptides that were detected (see Fig. S2 in the supplemental material). All members of the AOR family consist of a single subunit of approximately the same size (∼65 kDa) that contains a single tungsten atom bound by the pyranopterin cofactor (17–21). Given that XOR is of similar size and shows sequence similarity to the other AOR members, it is reasonable to assume it also contains a single tungsten atom. Hence, if XOR is the only tungsten-containing protein in the tungsten elution profile (Fig. 4), it represents ca. 1.9% of the cytoplasmic protein applied to the anion-exchange column.

FIG 4
  • Open in new tab
  • Download powerpoint
FIG 4

Elution profile of tungsten (red diamonds), molybdenum (purple triangles), and protein (blue squares) after fractionation of a cytoplasmic extract of C. bescii strain JWCB018 by anion-exchange chromatography. The cells were grown in LOD medium supplemented with 1 μM tungstate and 1 μM molybdate. Error bars for tungsten and molybdenum curves represent the SD (n = 3 technical replicates).

Phylogenetic analysis of the top 2000 BLAST hits for C. bescii XOR reveals that this enzyme is very distinct from the other five characterized members of the AOR family of enzymes. Moreover, as shown in Fig. 5, the XOR branch forms a very distinct and separate clade, a finding consistent with a function that is distinct from that of the other AOR family members. The characterized enzymes also fall in distinct clades (with WOR4 and WOR5 within a single clade). This analysis also reveals that there are at least three other large clades within the AOR family of enzymes about which little is known since there are no characterized representatives (Fig. 5). Further analysis of the gene synteny surrounding xor within the XOR clade shows that, although the gene cluster encoding proteins for pyranopterin biosynthesis and tungstate transport are not conserved, the gene encoding the polyferredoxin is always found adjacent to the XOR homolog (see Fig. S3 in the supplemental material). Hence, the polyferredoxin might be the electron carrier for XOR, or it could potentially be a subunit of a heterodimeric XOR enzyme. However, peptides attributable to the polyferredoxin (Athe_0820) were not detected in the fractions containing XOR (Athe_0821) after anion-exchange chromatography of a cell extract (Fig. 4).

FIG 5
  • Open in new tab
  • Download powerpoint
FIG 5

Phylogenetic tree of 2,000 AOR family homologs. The characterized enzymes from P. furiosus are indicated: formaldehyde ferredoxin oxidoreductase (FOR) (19), glyceraldehyde ferredoxin oxidoreductase (GAPOR) (18), aldehyde ferredoxin oxidoreductase (AOR) (17), and tungsten oxidoreductases of unknown function WOR4 and WOR5. The clade containing XOR is highlighted green to include homologs predicted across other species using the STRING database (20, 31). The protein sequences used to construct the tree were selected from protein BLAST hits of Athe_0821 against the NCBI database. The scale bar indicates the Jukes-Cantor distance of sequences on the tree.

To determine whether XOR was essential for growth of C. bescii on cellulose, an attempt was made to delete xor from the genome. Transformants for chromosomal integration of the xor knockout plasmid containing the pyrF marker were selected using uracil prototrophy, and counterselection for plasmid loss and gene deletion was performed using resistance to 5-fluoroorotic acid, as illustrated in Fig. S4 in the supplemental material. This same strategy was used to successfully knock out the gene encoding lactate dehydrogenase in the same genetic background strain (34). However, under these conditions, XOR deletion was not successful. Although recombination of the plasmid into the xor flanking region was verified, counterselection for plasmid loss resulted in reversion to the wild-type allele, and not xor deletion, in more than 200 screened isolates. Altogether, these results indicate that XOR plays a key role in C. bescii metabolism.

C. bescii contains a DMSOR homolog.In addition to the gene cluster encoding putative tungsten-containing XOR, polyferredoxin, pyranopterin synthesis, and tungstate transport, the C. bescii genome also contains a gene encoding a member of the dimethyl sulfoxide reductase (DMSOR) family of molybdoenzymes (Athe_1215), as well as an adjacent gene encoding the pyranopterin guanine dinucleotide synthesis gene mobA (Athe_1216). These two genes are located remotely from the XOR gene cluster, and they are also present as adjacent genes in all eight of the other available Caldicellulosiruptor genome sequences. The guanine dinucleotide form of pyranopterin is required for DMSOR family enzymes. Expression levels of Athe_1215 measured by qPCR show that it is expressed at ca. 10% of the level of the GAPDH gene (see Fig. S5 in the supplemental material). Microarray data suggest that Athe_1215 is not significantly regulated on any of the tested substrates (3). This DMSOR homolog was not detected via MS/MS analysis in either the Mo- or W-containing peaks of fractionated cell extract (Fig. 4), although this may be due to the lower expression level relative to the GAPDH gene compared with that of xor. Phylogenetic analysis reveals that the protein coded by this gene belongs to a subclass of the DMSOR family that is related to, but distinct from, well-characterized molybdoenzymes such as DMSOR, formate dehydrogenase, assimilatory and periplasmic nitrate reductases, trimethyl N-oxide reductase, and biotin sulfoxide reductase (see Fig. S6 in the supplemental material). This protein of unknown function does not resemble any of the characterized clades of DMSOR family enzymes and henceforth will be referred to as UDH for unknown dehydrogenase.

DISCUSSION

We show herein that C. bescii contains a gene cluster that encodes pyranopterin synthesis and tungstate transport. In addition, we demonstrate that C. bescii can heterologously express the P. furiosus tungsten-containing enzyme AOR at a high cellular concentration and with no apparent growth phenotype. The ability of C. bescii to express this AOR demonstrates that it has a very active tungsten utilization pathway, an important factor to consider for future engineering strategies in this organism. For example, the recently discovered alcohol production pathway from organic acids, involving AOR and an aldehyde dehydrogenase, AdhA (35), might be applicable in this organism and other Caldicellulosiruptor species.

Adjacent to the pyranonpterin biosynthetic gene cluster in the C. bescii genome is the gene encoding an AOR family enzyme for which function has yet to be determined and is therefore termed XOR. We show that xor is highly expressed in C. bescii, and it likely represents the major, if not the only, tungsten-containing enzyme within the cell. Phylogenetic analyses of the AOR family (Fig. 5) revealed that XOR is part of a unique clade distinct from the other characterized members. Surprisingly, this also revealed that there are at least three other major classes of the AOR family that remain to be functionally characterized, in addition to XOR. Of the five characterized members of the AOR family, only GAPOR has a well-defined physiological role. It replaces the conventional glycolytic enzyme GAPDH in some hyperthermophilic archaea and oxidizes glyeraldehyde-3-phosphate using ferredoxin rather than NAD as the electron acceptor (18). Of the others family members, AOR and the formaldehyde-oxidizing FOR are thought to function in peptide catabolism (19, 36). The functions of WOR4 and WOR5 remain unknown (20, 21).

Only a very few other microorganisms, including some acetogens and some ethanol- and phenylalanine-oxidizing anaerobes (12, 37, 38), also contain members of the AOR family, but these are all the prototypical type of AOR represented by P. furiosus AOR. This enzyme has broad substrate specificity and catalyzes the reversible oxidation of both aliphatic and aromatic aldehydes derived from amino acid metabolism. C. bescii is distinctive, since its AOR family member, XOR, does not utilize (at least in cytoplasmic extracts) the aldehydes oxidized by P. furiosus AOR, nor indeed by any other characterized member of the AOR family. Unfortunately, insight into the function of C. bescii XOR is not evident from microorganisms that contain XOR. As indicated in Fig. S3 in the supplemental material, they are quite diverse and include sulfate-reducing bacteria and methanogenic and sulfate-reducing archaea. The only common feature is that they are all anaerobic microorganisms. Hence, the designation of the C. bescii AOR homolog as XOR seems appropriate.

Although its role within the cell is as yet unknown, the conservation of XOR and its associated polyferredoxin across the genus Caldicellulosiruptor, together with its high expression level in C. bescii (∼2% of the cytoplasmic protein), suggest that this novel tungstoenzyme serves an important role in the primary metabolism of these cellulolytic species. Determining its function will likely have an important impact on future metabolic engineering studies of C. bescii, and studies to elucidate the substrate(s) utilized by XOR are under way.

ACKNOWLEDGMENTS

We thank Jeffrey Zurawski, Jonathan Conway, and Laura Lee for many helpful discussions and Daehwan Chung and Janet Westpheling for providing strains, plasmids, and protocols for genetic manipulation of C. bescii. We also acknowledge the University of Georgia Proteomics and Mass Spectrometry Facility for performing mass spectrometry analysis and computational support.

This research was supported by the U.S. Department of Energy's BioEnergy Science Center (BESC) through the Office of Biological and Environmental Research.

FOOTNOTES

    • Received 17 May 2015.
    • Accepted 30 July 2015.
    • Accepted manuscript posted online 14 August 2015.
  • Supplemental material for this article may be found at http://dx.doi.org/10.1128/AEM.01634-15.

  • Copyright © 2015, American Society for Microbiology. All Rights Reserved.

REFERENCES

  1. 1.↵
    1. Yang SJ,
    2. Kataeva I,
    3. Hamilton-Brehm SD,
    4. Engle NL,
    5. Tschaplinski TJ,
    6. Doeppke C,
    7. Davis M,
    8. Westpheling J,
    9. Adams MWW
    . 2009. Efficient degradation of lignocellulosic plant biomass, without pretreatment, by the thermophilic anaerobe “Anaerocellum thermophilum” DSM 6725. Appl Environ Microbiol 75:4762–4769. doi:10.1128/AEM.00236-09.
    OpenUrlAbstract/FREE Full Text
  2. 2.↵
    1. Blumer-Schuette SE,
    2. Giannone RJ,
    3. Zurawski JV,
    4. Ozdemir I,
    5. Ma Q,
    6. Yin YB,
    7. Xu Y,
    8. Kataeva I,
    9. Poole FL,
    10. Adams MWW,
    11. Hamilton-Brehm SD,
    12. Elkins JG,
    13. Larimer FW,
    14. Land ML,
    15. Hauser LJ,
    16. Cottingham RW,
    17. Hettich RL,
    18. Kelly RM
    . 2012. Caldicellulosiruptor core and pangenomes reveal determinants for noncellulosomal thermophilic deconstruction of plant biomass. J Bacteriol 194:4015–4028. doi:10.1128/JB.00266-12.
    OpenUrlAbstract/FREE Full Text
  3. 3.↵
    1. Kataeva I,
    2. Foston MB,
    3. Yang SJ,
    4. Pattathil S,
    5. Biswal A,
    6. Poole FL,
    7. Basen M,
    8. Rhaesa AM,
    9. Thomas TP,
    10. Azadi P,
    11. Olman V,
    12. Saffold TD,
    13. Mohler KE,
    14. Lewis DL,
    15. Doeppke C,
    16. Zeng Y,
    17. Tschaplinski T,
    18. York WS,
    19. Davis M,
    20. Mohnen D,
    21. Xu Y,
    22. Ragauskas AJ,
    23. Ding SY,
    24. Kelly RM,
    25. Hahn MG,
    26. Adams MWW
    . 2013. Carbohydrate and lignin are simultaneously solubilized from unpretreated switchgrass by microbial action at high temperature. Energ Environ Sci 6:2186–2195. doi:10.1039/c3ee40932e.
    OpenUrlCrossRefWeb of Science
  4. 4.↵
    1. Basen M,
    2. Rhaesa AM,
    3. Kataeva I,
    4. Prybol CJ,
    5. Scott IM,
    6. Poole FL,
    7. Adams MWW
    . 2014. Degradation of high loads of crystalline cellulose and of unpretreated plant biomass by the thermophilic bacterium Caldicellulosiruptor bescii. Bioresour Technol 152:384–392. doi:10.1016/j.biortech.2013.11.024.
    OpenUrlCrossRefPubMedWeb of Science
  5. 5.↵
    1. Chung D,
    2. Cha M,
    3. Guss AM,
    4. Westpheling J
    . 2014. Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii. Proc Natl Acad Sci U S A 111:8931–8936. doi:10.1073/pnas.1402210111.
    OpenUrlAbstract/FREE Full Text
  6. 6.↵
    1. Dam P,
    2. Kataeva I,
    3. Yang S-J,
    4. Zhou F,
    5. Yin Y,
    6. Chou W,
    7. Farris L,
    8. Poole I,
    9. Westpheling J,
    10. Hettich R,
    11. Giannone R,
    12. Lewis DL,
    13. Kelly R,
    14. Gilbert HJ,
    15. Henrissat B,
    16. Xu Y,
    17. Adams MWW
    . 2011. Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725. Nucleic Acids Res 39:3240–3254. doi:10.1093/nar/gkq1281.
    OpenUrlCrossRefPubMedWeb of Science
  7. 7.↵
    1. Hille R
    . 1996. The mononuclear molybdenum enzymes. Chem Rev 96:2757–2816. doi:10.1021/cr950061t.
    OpenUrlCrossRefPubMedWeb of Science
  8. 8.↵
    1. Hille R,
    2. Hall J,
    3. Basu P
    . 2014. The mononuclear molybdenum enzymes. Chem Rev 114:3963–4038. doi:10.1021/cr400443z.
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Makdessi K,
    2. Andreesen JR,
    3. Pich A
    . 2001. Tungstate uptake by a highly specific ABC transporter in Eubacterium acidaminophilum. J Biol Chem 276:24557–24564. doi:10.1074/jbc.M101293200.
    OpenUrlAbstract/FREE Full Text
  10. 10.↵
    1. Kletzin A,
    2. Adams MWW
    . 1996. Tungsten in biological systems. FEMS Microbiol Rev 18:5–63. doi:10.1111/j.1574-6976.1996.tb00226.x.
    OpenUrlCrossRefPubMedWeb of Science
  11. 11.↵
    1. Pushie MJ,
    2. Cotelesage JJ,
    3. George GN
    . 2014. Molybdenum and tungsten oxygen transferases: structural and functional diversity within a common active site motif. Metallomics 6:15–24. doi:10.1039/C3MT00177F.
    OpenUrlCrossRefPubMed
  12. 12.↵
    1. Andreesen JR,
    2. Makdessi K
    . 2008. Tungsten, the surprisingly positively acting heavy metal element for prokaryotes. Ann N Y Acad Sci 1125:215–229. doi:10.1196/annals.1419.003.
    OpenUrlCrossRefPubMed
  13. 13.↵
    1. Roy R,
    2. Adams MWW
    . 2002. Tungsten-dependent aldehyde oxidoreductase: a new family of enzymes containing the pterin cofactor. Metal Ions Biol Syst 39:673–697.
    OpenUrl
  14. 14.↵
    1. Chan MK,
    2. Mukund S,
    3. Kletzin A,
    4. Adams MW,
    5. Rees DC
    . 1995. Structure of a hyperthermophilic tungstopterin enzyme, aldehyde ferredoxin oxidoreductase. Science 267:1463–1469. doi:10.1126/science.7878465.
    OpenUrlAbstract/FREE Full Text
  15. 15.↵
    1. Johnson JL,
    2. Rajagopalan KV,
    3. Mukund S,
    4. Adams MW
    . 1993. Identification of molybdopterin as the organic component of the tungsten cofactor in four enzymes from hyperthermophilic Archaea. J Biol Chem 268:4848–4852.
    OpenUrlAbstract/FREE Full Text
  16. 16.↵
    1. Sevcenco A-M,
    2. Bevers LE,
    3. Pinkse MW,
    4. Krijger GC,
    5. Wolterbeek HT,
    6. Verhaert PD,
    7. Hagen WR,
    8. Hagedoorn P-L
    . 2010. Molybdenum incorporation in tungsten aldehyde oxidoreductase enzymes from Pyrococcus furiosus. J Bacteriol 192:4143–4152. doi:10.1128/JB.00270-10.
    OpenUrlAbstract/FREE Full Text
  17. 17.↵
    1. Mukund S,
    2. Adams MWW
    . 1991. The novel tungsten-iron-sulfur protein of the hyperthermophilic archaebacterium, Pyrococcus furiosus, is an aldehyde ferredoxin oxidoreductase: evidence for its participation in a unique glycolytic pathway. J Biol Chem 266:14208–14216.
    OpenUrlAbstract/FREE Full Text
  18. 18.↵
    1. Mukund S,
    2. Adams MWW
    . 1995. Glyceraldehyde-3-phosphate ferredoxin oxidoreductase, a novel tungsten-containing enzyme with a potential glycolytic role in the hyperthermophilic archaeon Pyrococcus furiosus. J Biol Chem 270:8389–8392. doi:10.1074/jbc.270.15.8389.
    OpenUrlAbstract/FREE Full Text
  19. 19.↵
    1. Roy R,
    2. Mukund S,
    3. Schut GJ,
    4. Dunn DM,
    5. Weiss R,
    6. Adams MWW
    . 1999. Purification and molecular characterization of the tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus: the third of a putative five-member tungstoenzyme family. J Bacteriol 181:1171–1180.
    OpenUrlAbstract/FREE Full Text
  20. 20.↵
    1. Roy R,
    2. Adams MWW
    . 2002. Characterization of a fourth tungsten-containing enzyme from the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol 184:6952–6956. doi:10.1128/JB.184.24.6952-6956.2002.
    OpenUrlAbstract/FREE Full Text
  21. 21.↵
    1. Bevers LE,
    2. Bol E,
    3. Hagedoorn P-L,
    4. Hagen WR
    . 2005. WOR5, a novel tungsten-containing aldehyde oxidoreductase from Pyrococcus furiosus with a broad substrate specificity. J Bacteriol 187:7056–7061. doi:10.1128/JB.187.20.7056-7061.2005.
    OpenUrlAbstract/FREE Full Text
  22. 22.↵
    1. Farkas J,
    2. Chung DW,
    3. Cha M,
    4. Copeland J,
    5. Grayeski P,
    6. Westpheling J
    . 2013. Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii. J Ind Microbiol Biotechnol 40:41–49. doi:10.1007/s10295-012-1202-1.
    OpenUrlCrossRefPubMed
  23. 23.↵
    1. Adams MWW,
    2. Holden JF,
    3. Menon AL,
    4. Schut GJ,
    5. Grunden AM,
    6. Hou C,
    7. Hutchins AM,
    8. Jenney FE,
    9. Kim C,
    10. Ma KS,
    11. Pan GL,
    12. Roy R,
    13. Sapra R,
    14. Story SV,
    15. Verhagen MFJM
    . 2001. Key role for sulfur in peptide metabolism and in regulation of three hydrogenases in the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol 183:716–724. doi:10.1128/JB.183.2.716-724.2001.
    OpenUrlAbstract/FREE Full Text
  24. 24.↵
    1. Farkas J,
    2. Stirrett K,
    3. Lipscomb GL,
    4. Nixon W,
    5. Scott RA,
    6. Adams MWW,
    7. Westpheling J
    . 2012. Recombinogenic properties of Pyrococcus furiosus strain COM1 enable rapid selection of targeted mutants. Appl Environ Microbiol 78:4669–4676. doi:10.1128/AEM.00936-12.
    OpenUrlAbstract/FREE Full Text
  25. 25.↵
    1. Chung D,
    2. Cha M,
    3. Farkas J,
    4. Westpheling J
    . 2013. Construction of a stable replicating shuttle vector for Caldicellulosiruptor species: use for extending genetic methodologies to other members of this genus. PLoS One 8:e62881. doi:10.1371/journal.pone.0062881.
    OpenUrlCrossRefPubMed
  26. 26.↵
    1. Chung D,
    2. Farkas J,
    3. Huddleston JR,
    4. Olivar E,
    5. Westpheling J
    . 2012. Methylation by a unique α-class N4-cytosine methyltransferase is required for DNA transformation of Caldicellulosiruptor bescii DSM6725. PLoS One 7:e43844. doi:10.1371/journal.pone.0043844.
    OpenUrlCrossRefPubMed
  27. 27.↵
    1. Lancaster WA,
    2. Menon AL,
    3. Scott I,
    4. Poole FL,
    5. Vaccaro BJ,
    6. Thorgersen MP,
    7. Geller J,
    8. Hazen TC,
    9. Hurt RA,
    10. Brown SD
    . 2014. Metallomics of two microorganisms relevant to heavy metal bioremediation reveal fundamental differences in metal assimilation and utilization. Metallomics 6:1004–1013. doi:10.1039/c4mt00050a.
    OpenUrlCrossRefPubMed
  28. 28.↵
    1. Heider J,
    2. Ma K,
    3. Adams MWW
    . 1995. Purification, characterization, and metabolic function of tungsten-containing aldehyde ferredoxin oxidoreductase from the hyperthermophilic and proteolytic archaeon Thermococcus strain ES-1. J Bacteriol 177:4757–4764.
    OpenUrlAbstract/FREE Full Text
  29. 29.↵
    1. Iobbi-Nivol C,
    2. Leimkuhler S
    . 2013. Molybdenum enzymes, their maturation and molybdenum cofactor biosynthesis in Escherichia coli. Biochim Biophys Acta 1827:1086–1101. doi:10.1016/j.bbabio.2012.11.007.
    OpenUrlCrossRefWeb of Science
  30. 30.↵
    1. Pierce E,
    2. Xie G,
    3. Barabote RD,
    4. Saunders E,
    5. Han CS,
    6. Detter JC,
    7. Richardson P,
    8. Brettin TS,
    9. Das A,
    10. Ljungdahl LG,
    11. Ragsdale SW
    . 2008. The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum). Environ Microbiol 10:2550–2573. doi:10.1111/j.1462-2920.2008.01679.x.
    OpenUrlCrossRefPubMedWeb of Science
  31. 31.↵
    1. Bevers LE,
    2. Hagedoorn P-L,
    3. Hagen WR
    . 2009. The bioinorganic chemistry of tungsten. Coordin Chem Rev 253:269–290. doi:10.1016/j.ccr.2008.01.017.
    OpenUrlCrossRefWeb of Science
  32. 32.↵
    1. Jeong KS,
    2. Ahn J,
    3. Khodursky AB
    . 2004. Spatial patterns of transcriptional activity in the chromosome of Escherichia coli. Genome Biol 5:R86. doi:10.1186/gb-2004-5-11-r86.
    OpenUrlCrossRefPubMed
  33. 33.↵
    1. Chung DW,
    2. Farkas J,
    3. Westpheling J
    . 2013. Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement. Biotechnol Biofuels 6:82. doi:10.1186/1754-6834-6-82.
    OpenUrlCrossRefPubMed
  34. 34.↵
    1. Cha M,
    2. Chung DW,
    3. Elkins JG,
    4. Guss AM,
    5. Westpheling J
    . 2013. Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass. Biotechnol Biofuels 6:85. doi:10.1186/1754-6834-6-85.
    OpenUrlCrossRefPubMed
  35. 35.↵
    1. Basen M,
    2. Schut GJ,
    3. Nguyen DM,
    4. Lipscomb GL,
    5. Benn RA,
    6. Prybol CJ,
    7. Vaccaro BJ,
    8. Poole FL,
    9. Kelly RM,
    10. Adams MWW
    . 2014. Single gene insertion drives bioalcohol production by a thermophilic archaeon. Proc Natl Acad Sci U S A 111:17618–17623. doi:10.1073/pnas.1413789111.
    OpenUrlAbstract/FREE Full Text
  36. 36.↵
    1. Hu Y,
    2. Faham S,
    3. Roy R,
    4. Adams MWW,
    5. Rees DC
    . 1999. Formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus: the 1.85 Å resolution crystal structure and its mechanistic implications. J Mol Biol 286:899–914. doi:10.1006/jmbi.1998.2488.
    OpenUrlCrossRefPubMedWeb of Science
  37. 37.↵
    1. Debnar-Daumler C,
    2. Seubert A,
    3. Schmitt G,
    4. Heider J
    . 2014. Simultaneous involvement of a tungsten-containing aldehyde: ferredoxin oxidoreductase and a phenylacetaldehyde dehydrogenase in anaerobic phenylalanine metabolism. J Bacteriol 196:483–492. doi:10.1128/JB.00980-13.
    OpenUrlAbstract/FREE Full Text
  38. 38.↵
    1. Schmidt A,
    2. Frensch M,
    3. Schleheck D,
    4. Schink B,
    5. Müller N
    . 2014. Degradation of acetaldehyde and its precursors by Pelobacter carbinolicus and P. acetylenicus. PLoS One 9:e115902. doi:10.1371/journal.pone.0115902.
    OpenUrlCrossRefPubMed
  39. 39.
    1. Yang SJ,
    2. Kataeva I,
    3. Wiegel J,
    4. Yin Y,
    5. Dam P,
    6. Xu Y,
    7. Westpheling J,
    8. Adams MWW
    . 2010. Classification of ‘Anaerocellum thermophilum’ strain DSM 6725 as Caldicellulosiruptor bescii sp. nov. Int J Syst Evol Microbiol 60:2011–2015. doi:10.1099/ijs.0.017731-0.
    OpenUrlCrossRefPubMed
View Abstract
PreviousNext
Back to top
Download PDF
Citation Tools
A New Class of Tungsten-Containing Oxidoreductase in Caldicellulosiruptor, a Genus of Plant Biomass-Degrading Thermophilic Bacteria
Israel M. Scott, Gabe M. Rubinstein, Gina L. Lipscomb, Mirko Basen, Gerrit J. Schut, Amanda M. Rhaesa, W. Andrew Lancaster, Farris L. Poole II, Robert M. Kelly, Michael W. W. Adams
Applied and Environmental Microbiology Sep 2015, 81 (20) 7339-7347; DOI: 10.1128/AEM.01634-15

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Applied and Environmental Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
A New Class of Tungsten-Containing Oxidoreductase in Caldicellulosiruptor, a Genus of Plant Biomass-Degrading Thermophilic Bacteria
(Your Name) has forwarded a page to you from Applied and Environmental Microbiology
(Your Name) thought you would be interested in this article in Applied and Environmental Microbiology.
Share
A New Class of Tungsten-Containing Oxidoreductase in Caldicellulosiruptor, a Genus of Plant Biomass-Degrading Thermophilic Bacteria
Israel M. Scott, Gabe M. Rubinstein, Gina L. Lipscomb, Mirko Basen, Gerrit J. Schut, Amanda M. Rhaesa, W. Andrew Lancaster, Farris L. Poole II, Robert M. Kelly, Michael W. W. Adams
Applied and Environmental Microbiology Sep 2015, 81 (20) 7339-7347; DOI: 10.1128/AEM.01634-15
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About AEM
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #AppEnvMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

Copyright © 2019 American Society for Microbiology | Privacy Policy | Website feedback

 

Print ISSN: 0099-2240; Online ISSN: 1098-5336