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Methods

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

Yu Jiang, Biao Chen, Chunlan Duan, Bingbing Sun, Junjie Yang, Sheng Yang
R. M. Kelly, Editor
Yu Jiang
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaShanghai Research Center of Industrial Biotechnology, Shanghai, China
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Biao Chen
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaShanghai Research Center of Industrial Biotechnology, Shanghai, China
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Chunlan Duan
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
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Bingbing Sun
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaShanghai Research Center of Industrial Biotechnology, Shanghai, China
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Junjie Yang
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaShanghai Research Center of Industrial Biotechnology, Shanghai, China
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Sheng Yang
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaShanghai Research Center of Industrial Biotechnology, Shanghai, ChinaShanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai, China
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R. M. Kelly
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DOI: 10.1128/AEM.04023-14
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Article Information

DOI 
https://doi.org/10.1128/AEM.04023-14
PubMed 
25636838

Published By 
American Society for Microbiology Journals
History 
  • Received December 10, 2014
  • Accepted January 17, 2015
  • Published online March 12, 2015.

Copyright & Usage 
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Editors and / or Reviewers 
  • R. M. Kelly, Editor

Article Versions

  • Accepted Manuscript version (January 30, 2015).
  • You are viewing the most recent version of this article.

Author Information

  1. Yu Jianga,b,
  2. Biao Chena,b,
  3. Chunlan Duana,
  4. Bingbing Suna,b,
  5. Junjie Yanga,b and
  6. Sheng Yanga,b,c
  1. aKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
  2. bShanghai Research Center of Industrial Biotechnology, Shanghai, China
  3. cShanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai, China
  • Address correspondence to Sheng Yang, syang{at}sibs.ac.cn.
  • Citation Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S. 2015. Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system. Appl Environ Microbiol 81:2506–2514. doi:10.1128/AEM.04023-14.

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Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
Yu Jiang, Biao Chen, Chunlan Duan, Bingbing Sun, Junjie Yang, Sheng Yang
Appl. Environ. Microbiol. Mar 2015, 81 (7) 2506-2514; DOI: 10.1128/AEM.04023-14

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Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
Yu Jiang, Biao Chen, Chunlan Duan, Bingbing Sun, Junjie Yang, Sheng Yang
Appl. Environ. Microbiol. Mar 2015, 81 (7) 2506-2514; DOI: 10.1128/AEM.04023-14
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