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Invertebrate Microbiology

Wolbachia Modulates Lipid Metabolism in Aedes albopictus Mosquito Cells

Jennifer C. Molloy, Ulf Sommer, Mark R. Viant, Steven P. Sinkins
P. D. Schloss, Editor
Jennifer C. Molloy
aNuffield Department of Medicine and Department of Zoology, University of Oxford, Oxford, United Kingdom
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Ulf Sommer
bNERC Biomolecular Analysis Facility—Metabolomics Node, School of Biosciences, University of Birmingham, Birmingham, United Kingdom
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Mark R. Viant
bNERC Biomolecular Analysis Facility—Metabolomics Node, School of Biosciences, University of Birmingham, Birmingham, United Kingdom
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Steven P. Sinkins
aNuffield Department of Medicine and Department of Zoology, University of Oxford, Oxford, United Kingdom
c Biomedical and Life Sciences, Lancaster University, Lancaster, United Kingdom
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P. D. Schloss
University of Michigan
Roles: Editor
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DOI: 10.1128/AEM.00275-16
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ABSTRACT

Certain strains of the intracellular endosymbiont Wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (DENV) by Aedes mosquitoes, and the mechanisms responsible are still not well understood. Direct infusion and liquid chromatography-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry-based lipidomics analyses were conducted using Aedes albopictus Aa23 cells that were infected with the wMel and wMelPop strains of Wolbachia in comparison to uninfected Aa23-T cells. Substantial shifts in the cellular lipid profile were apparent in the presence of Wolbachia. Most significantly, almost all sphingolipid classes were depleted, and some reductions in diacylglycerols and phosphatidylcholines were also observed. These lipid classes have previously been shown to be selectively enriched in DENV-infected mosquito cells, suggesting that Wolbachia may produce a cellular lipid environment that is antagonistic to viral replication. The data improve our understanding of the intracellular interactions between Wolbachia and mosquitoes.

IMPORTANCE Mosquitoes transmit a variety of important viruses to humans, such as dengue virus and Zika virus. Certain strains of the intracellular bacterial genus called Wolbachia found in or introduced into mosquitoes can block the transmission of viruses, including dengue virus, but the mechanisms responsible are not well understood. We found substantial shifts in the cellular lipid profiles in the presence of these bacteria. Some lipid classes previously shown to be enriched in dengue virus-infected mosquito cells were depleted in the presence of Wolbachia, suggesting that Wolbachia may produce a cellular lipid environment that inhibits mosquito-borne viruses.

FOOTNOTES

    • Received 27 January 2016.
    • Accepted 10 March 2016.
    • Accepted manuscript posted online 18 March 2016.
  • Address correspondence to Steven P. Sinkins, s.sinkins{at}lancaster.ac.uk.
  • J.C.M. and U.S. contributed equally to this article.

  • Citation Molloy JC, Sommer U, Viant MR, Sinkins SP. 2016. Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells. Appl Environ Microbiol 82:3109–3120. doi:10.1128/AEM.00275-16.

  • Supplemental material for this article may be found at http://dx.doi.org/10.1128/AEM.00275-16.

  • Copyright © 2016 Molloy et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Wolbachia Modulates Lipid Metabolism in Aedes albopictus Mosquito Cells
Jennifer C. Molloy, Ulf Sommer, Mark R. Viant, Steven P. Sinkins
Applied and Environmental Microbiology May 2016, 82 (10) 3109-3120; DOI: 10.1128/AEM.00275-16

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Wolbachia Modulates Lipid Metabolism in Aedes albopictus Mosquito Cells
Jennifer C. Molloy, Ulf Sommer, Mark R. Viant, Steven P. Sinkins
Applied and Environmental Microbiology May 2016, 82 (10) 3109-3120; DOI: 10.1128/AEM.00275-16
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