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Environmental Microbiology | Spotlight

Generation and Characterization of Indoor Fungal Aerosols for Inhalation Studies

Anne Mette Madsen, Søren T. Larsen, Ismo K. Koponen, Kirsten I. Kling, Afnan Barooni, Dorina Gabriela Karottki, Kira Tendal, Peder Wolkoff
A. A. Brakhage, Editor
Anne Mette Madsen
aThe National Research Centre for the Working Environment, Copenhagen, Denmark
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Søren T. Larsen
aThe National Research Centre for the Working Environment, Copenhagen, Denmark
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Ismo K. Koponen
aThe National Research Centre for the Working Environment, Copenhagen, Denmark
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Kirsten I. Kling
aThe National Research Centre for the Working Environment, Copenhagen, Denmark
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Afnan Barooni
aThe National Research Centre for the Working Environment, Copenhagen, Denmark
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Dorina Gabriela Karottki
bSection of Environmental Health, Department of Public Health, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Kira Tendal
aThe National Research Centre for the Working Environment, Copenhagen, Denmark
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Peder Wolkoff
aThe National Research Centre for the Working Environment, Copenhagen, Denmark
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A. A. Brakhage
HKI and University of Jena
Roles: Editor
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DOI: 10.1128/AEM.04063-15
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ABSTRACT

In the indoor environment, people are exposed to several fungal species. Evident dampness is associated with increased respiratory symptoms. To examine the immune responses associated with fungal exposure, mice are often exposed to a single species grown on an agar medium. The aim of this study was to develop an inhalation exposure system to be able to examine responses in mice exposed to mixed fungal species aerosolized from fungus-infested building materials. Indoor airborne fungi were sampled and cultivated on gypsum boards. Aerosols were characterized and compared with aerosols in homes. Aerosols containing 107 CFU of fungi/m3 air were generated repeatedly from fungus-infested gypsum boards in a mouse exposure chamber. Aerosols contained Aspergillus nidulans, Aspergillus niger, Aspergillus ustus, Aspergillus versicolor, Chaetomium globosum, Cladosporium herbarum, Penicillium brevicompactum, Penicillium camemberti, Penicillium chrysogenum, Penicillium commune, Penicillium glabrum, Penicillium olsonii, Penicillium rugulosum, Stachybotrys chartarum, and Wallemia sebi. They were all among the most abundant airborne species identified in 28 homes. Nine species from gypsum boards and 11 species in the homes are associated with water damage. Most fungi were present as single spores, but chains and clusters of different species and fragments were also present. The variation in exposure level during the 60 min of aerosol generation was similar to the variation measured in homes. Through aerosolization of fungi from the indoor environment, cultured on gypsum boards, it was possible to generate realistic aerosols in terms of species composition, concentration, and particle sizes. The inhalation-exposure system can be used to study responses to indoor fungi associated with water damage and the importance of fungal species composition.

  • Copyright © 2016 Madsen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Generation and Characterization of Indoor Fungal Aerosols for Inhalation Studies
Anne Mette Madsen, Søren T. Larsen, Ismo K. Koponen, Kirsten I. Kling, Afnan Barooni, Dorina Gabriela Karottki, Kira Tendal, Peder Wolkoff
Applied and Environmental Microbiology Apr 2016, 82 (8) 2479-2493; DOI: 10.1128/AEM.04063-15

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Generation and Characterization of Indoor Fungal Aerosols for Inhalation Studies
Anne Mette Madsen, Søren T. Larsen, Ismo K. Koponen, Kirsten I. Kling, Afnan Barooni, Dorina Gabriela Karottki, Kira Tendal, Peder Wolkoff
Applied and Environmental Microbiology Apr 2016, 82 (8) 2479-2493; DOI: 10.1128/AEM.04063-15
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