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Food Microbiology

Relationship of Enhanced Butyrate Production by Colonic Butyrate-Producing Bacteria to Immunomodulatory Effects in Normal Mice Fed an Insoluble Fraction of Brassica rapa L.

Sachi Tanaka, Kana Yamamoto, Kazuki Yamada, Kanon Furuya, Yutaka Uyeno
T. E. Besser, Editor
Sachi Tanaka
aAcademic Assembly (Institute of Agriculture), Shinshu University, Minamiminowa, Nagano, Japan
bFaculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan
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Kana Yamamoto
bFaculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan
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Kazuki Yamada
bFaculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan
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Kanon Furuya
bFaculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan
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Yutaka Uyeno
aAcademic Assembly (Institute of Agriculture), Shinshu University, Minamiminowa, Nagano, Japan
bFaculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan
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T. E. Besser
Washington State University
Roles: Editor
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DOI: 10.1128/AEM.03343-15
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ABSTRACT

This study was performed to determine the effects of feeding a fiber-rich fraction of Brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (SCFA) production in normal mice. The boiled-water-insoluble fraction of Brassica rapa L. (nozawana), which consists mainly of dietary fiber, was chosen as a test material. A total of 31 male C57BL/6J mice were divided into two groups and housed in a specific-pathogen-free facility. The animals were fed either a control diet or the control diet plus the insoluble B. rapa L. fraction for 2 weeks and sacrificed to determine microbiological and SCFA profiles in lower-gut samples and immunological molecules. rRNA-based quantification indicated that the relative population of Bacteroidetes was markedly lower in the colon samples of the insoluble B. rapa L. fraction-fed group than that in the controls. Populations of the Eubacterium rectale group and Faecalibacterium prausnitzii, both of which are representative butyrate-producing bacteria, doubled after 2 weeks of fraction intake, accompanying a marginal increase in the proportion of colonic butyrate. In addition, feeding with the fraction significantly increased levels of the anti-inflammatory cytokine interleukin-10 (IL-10) and tended to increase splenic regulatory T cell numbers but significantly reduced the population of cells expressing activation markers. We demonstrated that inclusion of the boiled-water-insoluble fraction of B. rapa L. can alter the composition of the gut microbiota to decrease the numbers of Bacteroidetes and to increase the numbers of butyrate-producing bacteria, either of which may be involved in the observed shift in the production of splenic IL-10.

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Relationship of Enhanced Butyrate Production by Colonic Butyrate-Producing Bacteria to Immunomodulatory Effects in Normal Mice Fed an Insoluble Fraction of Brassica rapa L.
Sachi Tanaka, Kana Yamamoto, Kazuki Yamada, Kanon Furuya, Yutaka Uyeno
Applied and Environmental Microbiology Apr 2016, 82 (9) 2693-2699; DOI: 10.1128/AEM.03343-15

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Relationship of Enhanced Butyrate Production by Colonic Butyrate-Producing Bacteria to Immunomodulatory Effects in Normal Mice Fed an Insoluble Fraction of Brassica rapa L.
Sachi Tanaka, Kana Yamamoto, Kazuki Yamada, Kanon Furuya, Yutaka Uyeno
Applied and Environmental Microbiology Apr 2016, 82 (9) 2693-2699; DOI: 10.1128/AEM.03343-15
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