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Biotechnology

Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4

Nicolaus A. Herman, Jeffrey Li, Ripika Bedi, Barbara Turchi, Xiaoji Liu, Michael J. Miller, Wenjun Zhang
Robert M. Kelly, Editor
Nicolaus A. Herman
aDepartment of Chemical and Biomolecular Engineering, University of California—Berkeley, Berkeley, California, USA
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Jeffrey Li
aDepartment of Chemical and Biomolecular Engineering, University of California—Berkeley, Berkeley, California, USA
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Ripika Bedi
bDepartment of Chemistry, University of California—Berkeley, Berkeley, California, USA
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Barbara Turchi
cDepartment of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
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Xiaoji Liu
cDepartment of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
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Michael J. Miller
cDepartment of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
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  • ORCID record for Michael J. Miller
Wenjun Zhang
aDepartment of Chemical and Biomolecular Engineering, University of California—Berkeley, Berkeley, California, USA
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Robert M. Kelly
North Carolina State University
Roles: Editor
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DOI: 10.1128/AEM.02942-16
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Article Information

DOI 
https://doi.org/10.1128/AEM.02942-16
PubMed 
27836845

Published By 
American Society for Microbiology Journals
History 
  • Received October 22, 2016
  • Accepted November 1, 2016
  • Published online December 30, 2016.

Copyright & Usage 
Copyright © 2016 American Society for Microbiology. All Rights Reserved .
Editors and / or Reviewers 
  • Robert M. Kelly, Editor, North Carolina State University

Article Versions

  • Accepted Manuscript version (November 11, 2016).
  • You are viewing the most recent version of this article.

Author Information

  1. Nicolaus A. Hermana,
  2. Jeffrey Lia,
  3. Ripika Bedib,
  4. Barbara Turchic*,
  5. Xiaoji Liuc,
  6. Michael J. Millerc and
  7. Wenjun Zhanga
  1. aDepartment of Chemical and Biomolecular Engineering, University of California—Berkeley, Berkeley, California, USA
  2. bDepartment of Chemistry, University of California—Berkeley, Berkeley, California, USA
  3. cDepartment of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
  • Address correspondence to Wenjun Zhang, wjzhang{at}berkeley.edu.
  • ↵* Present address: Barbara Turchi, Department of Veterinary Science, University of Pisa, Pisa, Italy.

  • J.L. and R.B. contributed equally to this work.

  • Citation Herman NA, Li J, Bedi R, Turchi B, Liu X, Miller MJ, Zhang W. 2017. Development of a high-efficiency transformation method and implementation of rational metabolic engineering for the industrial butanol hyperproducer Clostridium saccharoperbutylacetonicum strain N1-4. Appl Environ Microbiol 83:e02942-16. https://doi.org/10.1128/AEM.02942-16 .

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Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4
Nicolaus A. Herman, Jeffrey Li, Ripika Bedi, Barbara Turchi, Xiaoji Liu, Michael J. Miller, Wenjun Zhang
Applied and Environmental Microbiology Dec 2016, 83 (2) e02942-16; DOI: 10.1128/AEM.02942-16

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Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4
Nicolaus A. Herman, Jeffrey Li, Ripika Bedi, Barbara Turchi, Xiaoji Liu, Michael J. Miller, Wenjun Zhang
Applied and Environmental Microbiology Dec 2016, 83 (2) e02942-16; DOI: 10.1128/AEM.02942-16
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KEYWORDS

biofuels
Butanols
Clostridium
metabolic engineering
ABE fermentation
Clostridium
biofuel
genetics

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