Article Figures & Data
Figures
- FIG 1
Colony morphology of B. humptydooensis sp. nov. MSMB43T. Cultures were grown on Ashdown's agar (a) or on Standard I nutrient agar (b).
- FIG 2
Core genome phylogeny of B. humptydooensis sp. nov. SNPs from the comparison of four B. humptydooensis sp. nov. genomes and representatives of the other closely related species were used to reconstruct the phylogenetic relationships. Genomes from this study are shown in bold and assembly numbers are provided inside parentheses. Numbers at nodes indicate bootstrap support values. Collapsed nodes are shown in gray.
Tables
- TABLE 1
Phenotypic characteristics of B. humptydooensis sp. nov. and closely related species within the B. pseudomallei group
Biochemical reaction Characteristic (compound present in medium or assimilated by strain) B. pseudomallei K96243a B. thailandensis E264T B. humptydooensis sp. nov. MSMB43T B. humptydooensis sp. nov. MSMB121 B. humptydooensis sp. nov. MSMB122 Tryptophan + − − − − Arginine + − + − − Esculin − + + + + PNPG − − − − + Arabinose assimilation − + + + + Maltose assimilation − + − − − ↵a Data for B. pseudomallei K96243 were obtained from Wuthiekanun et al. (29).
- TABLE 2
Summary of MICs determined in triplicate by the broth microdilution method
Antimicrobial substance MIC (mg/liter) MSMB43T MSMB121 MSMB122 Amoxicillin-clavulanic acida 32/16 32/16 32/16 Ceftazidime 4 2 4 Imipenem 0.5 0.5 0.5 Rifampin >8 >8 8 Chloramphenicol 8 4 4 Trimethoprim-sulfamethoxazole 0.5/9.5 ≤0.25/4.75 ≤0.25/4.75 Streptomycin >32 >32 >32 Gentamicin 32 32 >32 Doxycycline 1 1 1 Tigecycline 2 4 4 Ciprofloxacin 0.5 1 1 Levofloxacin 0.5 1 1 ↵a Resistance was observed, based upon the CLSI breakpoints of B. pseudomallei.
- TABLE 3
Whole-genome dataa for B. pseudomallei group organisms
Species and strain GC content (%) Genome size (Mb) No. of CDSc B. humptydooensis sp. nov. MSMB43T 67.1 7.3b 6,324 B. humptydooensis sp. nov. MSMB121 67.5 6.7 5,795 B. humptydooensis sp. nov. MSMB122 67.5 6.8 5,845 B. thailandensis E264T 67.6 6.7 5,652 B. oklahomensis C6786T 66.9 7.1 6,097 B. pseudomallei K96243 68.1 7.2 5,948 B. mallei ATCC 23344T 68.5 5.8 5,006 - TABLE 4
GGDC and ANI values for whole-genome sequence similarities
a Genome-to-genome distance calculations (with confidence intervals) are shown in the bottom left half of the matrix (below the line of identity, i.e., the line formed by blank cells for comparisons of strains with themselves); average nucleotide identities are shown in the top right half of the matrix. Values in shaded boxes represent values above the similarity threshold that defines members of the same species.
Supplemental material
- Supplemental file 1 -
Detailed methods: MALDI-TOF and FAME profile analysis; dendrogram demonstrating strain relatedness revealed by MALDI-TOF analysis (Fig. S1); dendrogram demonstrating the relatedness of fatty acid compositions in B. humptydooensis sp. nov. and closely related species (Fig. S2); 16S maximum-likelihood phylogeny using both copies of the 16S rRNA gene from each B. humptydooensis sp. nov. strain (Fig. S3); maximum-likelihood phylogeny of the recA sequence using 193 sequences (Fig. S4).
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- Supplemental file 1 -
