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Methods

Sybr Green- and TaqMan-Based Quantitative PCR Approaches Allow Assessment of the Abundance and Relative Distribution of Frankia Clusters in Soils

Seifeddine Ben Tekaya, Abirama Sundari Ganesan, Trina Guerra, Jeffrey O. Dawson, Michael R. J. Forstner, Dittmar Hahn
Claire Vieille, Editor
Seifeddine Ben Tekaya
aTexas State University, Department of Biology, San Marcos, Texas, USA
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Abirama Sundari Ganesan
aTexas State University, Department of Biology, San Marcos, Texas, USA
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Trina Guerra
aTexas State University, Department of Biology, San Marcos, Texas, USA
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Jeffrey O. Dawson
bUniversity of Illinois at Urbana-Champaign, Department of Natural Resources and Environmental Sciences, Urbana, Illinois, USA
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Michael R. J. Forstner
aTexas State University, Department of Biology, San Marcos, Texas, USA
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Dittmar Hahn
aTexas State University, Department of Biology, San Marcos, Texas, USA
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Claire Vieille
Michigan State University
Roles: Editor
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DOI: 10.1128/AEM.02833-16
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Article Information

DOI 
https://doi.org/10.1128/AEM.02833-16
PubMed 
27986724

Published By 
American Society for Microbiology Journals
History 
  • Received October 12, 2016
  • Accepted December 9, 2016
  • Published online February 15, 2017.

Copyright & Usage 
Copyright © 2017 American Society for Microbiology. All Rights Reserved .
Editors and / or Reviewers 
  • Claire Vieille, Editor, Michigan State University

Article Versions

  • Accepted Manuscript version (December 16, 2016).
  • You are viewing the most recent version of this article.

Author Information

  1. Seifeddine Ben Tekayaa,
  2. Abirama Sundari Ganesana,
  3. Trina Guerraa,
  4. Jeffrey O. Dawsonb,
  5. Michael R. J. Forstnera and
  6. Dittmar Hahna
  1. aTexas State University, Department of Biology, San Marcos, Texas, USA
  2. bUniversity of Illinois at Urbana-Champaign, Department of Natural Resources and Environmental Sciences, Urbana, Illinois, USA
  • Address correspondence to Dittmar Hahn, dh49{at}txstate.edu.
  • Citation Ben Tekaya S, Ganesan AS, Guerra T, Dawson JO, Forstner MRJ, Hahn D. 2017. Sybr Green- and TaqMan-based quantitative PCR approaches allow assessment of the abundance and relative distribution of Frankia clusters in soils. Appl Environ Microbiol 83:e02833-16. https://doi.org/10.1128/AEM.02833-16 .

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Sybr Green- and TaqMan-Based Quantitative PCR Approaches Allow Assessment of the Abundance and Relative Distribution of Frankia Clusters in Soils
Seifeddine Ben Tekaya, Abirama Sundari Ganesan, Trina Guerra, Jeffrey O. Dawson, Michael R. J. Forstner, Dittmar Hahn
Applied and Environmental Microbiology Feb 2017, 83 (5) e02833-16; DOI: 10.1128/AEM.02833-16

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Sybr Green- and TaqMan-Based Quantitative PCR Approaches Allow Assessment of the Abundance and Relative Distribution of Frankia Clusters in Soils
Seifeddine Ben Tekaya, Abirama Sundari Ganesan, Trina Guerra, Jeffrey O. Dawson, Michael R. J. Forstner, Dittmar Hahn
Applied and Environmental Microbiology Feb 2017, 83 (5) e02833-16; DOI: 10.1128/AEM.02833-16
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KEYWORDS

Frankia
Multigene Family
Real-Time Polymerase Chain Reaction
soil microbiology
alder
birch
qPCR
quantification
saprotrophic
soil

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