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Biotechnology

Improvement of Euglena gracilis Paramylon Production through a Cocultivation Strategy with the Indole-3-Acetic Acid-Producing Bacterium Vibrio natriegens

Jee Young Kim, Jeong-Joo Oh, Min Seo Jeon, Gyu-Hyeok Kim, Yoon-E Choi
Haruyuki Atomi, Editor
Jee Young Kim
aDivision of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea
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Jeong-Joo Oh
aDivision of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea
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Min Seo Jeon
aDivision of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea
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Gyu-Hyeok Kim
aDivision of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea
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Yoon-E Choi
aDivision of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea
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Haruyuki Atomi
Kyoto University
Roles: Editor
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DOI: 10.1128/AEM.01548-19
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ABSTRACT

We investigated the putative effects on the growth and paramylon production of Euglena gracilis of cocultivation with Vibrio natriegens. E. gracilis heterotrophically cocultivated with V. natriegens displayed significant increases in biomass productivity and paramylon content. In addition, the effects of the bacterial inoculum density and the timing of inoculation on the growth of E. gracilis were examined, to determine the optimal conditions for cocultivation. With the optimal deployment of V. natriegens, biomass productivity and paramylon content were increased by more than 20% and 35%, respectively, compared to those in axenic E. gracilis cultures. Interestingly, indole-3-acetic acid biosynthesized by V. natriegens was responsible for these enhancements of E. gracilis. The morphology of cocultured E. gracilis cells was assessed. Paramylon granules extracted from the cocultivation were significantly larger than those from axenic culture. Our study showed that screening for appropriate bacteria and subsequent cocultivation with E. gracilis represented an effective way to enhance biomass and metabolite production.

IMPORTANCE Euglena gracilis has attracted special interest due to its ability to excessively accumulate paramylon. Paramylon is a linear β-1,3-glucan polysaccharide that is the principal polymer for energy storage in E. gracilis. The polysaccharide features high bioactive functionality in the immune system. This study explored a new method to enhance the production of paramylon by E. gracilis, through cocultivation with the indole-3-acetic acid-producing bacterium Vibrio natriegens. The enhanced production was achieved indirectly with the phytohormone-producing bacteria, instead of direct application of the hormone. The knowledge obtained in this study furthers the understanding of the effects of V. natriegens on the growth and physiology of E. gracilis.

FOOTNOTES

    • Received 10 July 2019.
    • Accepted 15 July 2019.
    • Accepted manuscript posted online 19 July 2019.
  • Supplemental material for this article may be found at https://doi.org/10.1128/AEM.01548-19.

  • Copyright © 2019 American Society for Microbiology.

All Rights Reserved.

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Improvement of Euglena gracilis Paramylon Production through a Cocultivation Strategy with the Indole-3-Acetic Acid-Producing Bacterium Vibrio natriegens
Jee Young Kim, Jeong-Joo Oh, Min Seo Jeon, Gyu-Hyeok Kim, Yoon-E Choi
Applied and Environmental Microbiology Sep 2019, 85 (19) e01548-19; DOI: 10.1128/AEM.01548-19

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Improvement of Euglena gracilis Paramylon Production through a Cocultivation Strategy with the Indole-3-Acetic Acid-Producing Bacterium Vibrio natriegens
Jee Young Kim, Jeong-Joo Oh, Min Seo Jeon, Gyu-Hyeok Kim, Yoon-E Choi
Applied and Environmental Microbiology Sep 2019, 85 (19) e01548-19; DOI: 10.1128/AEM.01548-19
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KEYWORDS

cocultivation
Euglena gracilis
IAA
paramylon
Vibrio natriegens

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