Article Figures & Data
Figures
- FIG 1
(A) Experimental set up of FISS chambers during the outdoor growth trials on the roof of the Life Sciences Building East at Arizona State University (Tempe, AZ) during October to November 2018. (B) Automated scale-up of FISS chamber to 1.5-m plastic pools with cotton fitted sheet covering. (C) General schematic of FISS system setup.
- FIG 2
Growth dynamics (mean biomass ± standard deviation [SD], n = 12) of FISS-grown Microcoleus cultures. Shaded lines indicate maximum average Chl a values (±SD) obtained with the floating cellulose tissue method (32) for the same strains at peak biomass.
- FIG 3
(A) Visual aspect of mixed-community, outdoor-incubated FISS-grown biocrusts under different shade treatments. (B) Chlorophyll a concentrations in mixed-community FISS trials given in mg Chl a m−2. Shown are means (n = 5) ± standard deviations. P values are the result of an ANOVA between treatment Chl a values (95% confidence interval).
- FIG 4
(A) NMDS ordination of Bray-Curtis pairwise distance computed on the cyanobacterial sequence composition of remnant cold desert biocrust samples (field), native soil (substrate), or FISS-grown mixed-community inoculum (shaded/nonshaded), with 95% confidence ellipses drawn for each with a stress value of 0.049. Cyanobacterial communities show significant differences between treatments (PERMANOVA, P < 0.001). (B) Cyanobacterial abundance and community structure of cold desert biocrusts of FISS-grown inoculum under different shade treatments, as determined by high-throughput 16S rRNA gene analysis coupled to qPCR. Note: “Microcoleus steenstrupii” corresponds phylogenetically to a complex of several genus-level clades (19), these clades are distinguished in the figure.
- FIG 5
(A) NMDS ordination of Bray-Curtis pairwise distance computed on the cyanobacterial sequence composition of remnant hot desert biocrust samples (field), native soil (substrate), or FISS-grown mixed-community inoculum (shaded/nonshaded), with 95% confidence ellipses drawn for each with a stress value of 0.049. Cyanobacterial communities show significant differences between treatments (PERMANOVA, P < 0.001). (B) Cyanobacterial abundance and community structure of hot desert biocrusts of FISS-grown inoculum under different shade treatments, as determined by high-throughput 16S rRNA gene analysis coupled to qPCR. Note: “Microcoleus steenstrupii” corresponds phylogenetically to a complex of several genus-level clades (19); these clades are distinguished in the figure.
Additional Files
Supplemental material
- Supplemental file 1 -
Tables S1 and S2, Fig. S1 to S7
PDF, 2.0M
- Supplemental file 1 -
