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Biotechnology

Changes in the Microbiome of Mariculture Feed Organisms after Treatment with a Potentially Probiotic Strain of Phaeobacter inhibens

Karen K. Dittmann, Bastian Barker Rasmussen, Jette Melchiorsen, Eva C. Sonnenschein, Lone Gram, Mikkel Bentzon-Tilia
Shuang-Jiang Liu, Editor
Karen K. Dittmann
aDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark
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Bastian Barker Rasmussen
aDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark
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Jette Melchiorsen
aDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark
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Eva C. Sonnenschein
aDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark
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Lone Gram
aDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark
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Mikkel Bentzon-Tilia
aDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark
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Shuang-Jiang Liu
Chinese Academy of Sciences
Roles: Editor
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DOI: 10.1128/AEM.00499-20
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ABSTRACT

The Phaeobacter genus has been explored as probiotics in mariculture as a sustainable strategy for the prevention of bacterial infections. Its antagonistic effect against common fish pathogens is predominantly due to the production of the antibacterial compound tropodithietic acid (TDA), and TDA-producing strains have repeatedly been isolated from mariculture environments. Despite many in vitro trials targeting pathogens, little is known about its impact on host-associated microbiomes in mariculture. Hence, the purpose of this study was to investigate how the addition of a TDA-producing Phaeobacter inhibens strain affects the microbiomes of live feed organisms and fish larvae. We used 16S rRNA gene sequencing to characterize the bacterial diversity associated with live feed microalgae (Tetraselmis suecica), live feed copepod nauplii (Acartia tonsa), and turbot (Scophthalmus maximus) eggs/larvae. The microbial communities were unique to the three organisms investigated, and the addition of the probiotic bacterium had various effects on the diversity and richness of the microbiomes. The structure of the live feed microbiomes was significantly changed, while no effect was seen on the community structure associated with turbot larvae. The changes were seen primarily in particular taxa. The Rhodobacterales order was indigenous to all three microbiomes and decreased in relative abundance when P. inhibens was introduced in the copepod and turbot microbiomes, while it was unaffected in the microalgal microbiome. Altogether, the study demonstrates that the addition of P. inhibens in higher concentrations, as part of a probiotic regime, does not appear to cause major imbalances in the microbiome, but the effects were specific to closely related taxa.

IMPORTANCE This work is an essential part of the risk assessment of the application of roseobacters as probiotics in mariculture. It provides insights into the impact of TDA-producing Phaeobacter inhibens on the commensal bacteria related to mariculture live feed and fish larvae. Also, the study provides a sequencing-based characterization of the microbiomes related to mariculture-relevant microalga, copepods, and turbot larvae.

FOOTNOTES

    • Received 27 February 2020.
    • Accepted 3 May 2020.
    • Accepted manuscript posted online 8 May 2020.
  • Supplemental material is available online only.

  • Copyright © 2020 American Society for Microbiology.

All Rights Reserved.

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Changes in the Microbiome of Mariculture Feed Organisms after Treatment with a Potentially Probiotic Strain of Phaeobacter inhibens
Karen K. Dittmann, Bastian Barker Rasmussen, Jette Melchiorsen, Eva C. Sonnenschein, Lone Gram, Mikkel Bentzon-Tilia
Applied and Environmental Microbiology Jul 2020, 86 (14) e00499-20; DOI: 10.1128/AEM.00499-20

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Changes in the Microbiome of Mariculture Feed Organisms after Treatment with a Potentially Probiotic Strain of Phaeobacter inhibens
Karen K. Dittmann, Bastian Barker Rasmussen, Jette Melchiorsen, Eva C. Sonnenschein, Lone Gram, Mikkel Bentzon-Tilia
Applied and Environmental Microbiology Jul 2020, 86 (14) e00499-20; DOI: 10.1128/AEM.00499-20
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KEYWORDS

16S rRNA amplicon sequencing
Phaeobacter
Roseobacter
aquaculture
microbial community composition
microbiome
tropodithietic acid

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