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Food Microbiology

Microorganisms Move a Short Distance into an Almond Orchard from an Adjacent Upwind Poultry Operation

Christopher G. Theofel, Thomas R. Williams, Eduardo Gutierrez, Gordon R. Davidson, Michele Jay-Russell, Linda J. Harris
Donald W. Schaffner, Editor
Christopher G. Theofel
aDepartment of Food Science and Technology, University of California, Davis, Davis, California, USA
bWestern Center for Food Safety, University of California, Davis, Davis, California, USA
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Thomas R. Williams
aDepartment of Food Science and Technology, University of California, Davis, Davis, California, USA
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Eduardo Gutierrez
aDepartment of Food Science and Technology, University of California, Davis, Davis, California, USA
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Gordon R. Davidson
aDepartment of Food Science and Technology, University of California, Davis, Davis, California, USA
bWestern Center for Food Safety, University of California, Davis, Davis, California, USA
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Michele Jay-Russell
bWestern Center for Food Safety, University of California, Davis, Davis, California, USA
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Linda J. Harris
aDepartment of Food Science and Technology, University of California, Davis, Davis, California, USA
bWestern Center for Food Safety, University of California, Davis, Davis, California, USA
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  • ORCID record for Linda J. Harris
Donald W. Schaffner
Rutgers, The State University of New Jersey
Roles: Editor
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DOI: 10.1128/AEM.00573-20
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This article has a correction. Please see:

  • Correction for Theofel et al., “Microorganisms Move a Short Distance into an Almond Orchard from an Adjacent Upwind Poultry Operation” - September 17, 2020

ABSTRACT

Over a 2-year period, drag swabs of orchard soil surface and air, soil, and almond leaf samples were collected in an almond orchard adjacent to (35 m from the first row of trees) and downwind from a poultry operation and in two almond orchards (controls) that were surrounded by other orchards. Samples were evaluated for aerobic plate count, generic Escherichia coli, other coliforms, the presence of Salmonella, bacterial community structure (analyzed through sequencing of the 16S rRNA gene), and amounts of dry solids (dust) on leaf surfaces on trees 0, 60, and 120 m into each orchard. E. coli was isolated from 41 of 206 (20%) and 1 of 207 (0.48%) air samples in the almond-poultry and control orchards, respectively. Salmonella was not isolated from any of the 529 samples evaluated. On average, the amount of dry solids on leaves collected from trees closest to the poultry operation was more than 2-fold greater than from trees 120 m into the orchard or from any of the trees in the control orchards. Members of the family Staphylococcaceae—often associated with poultry—were, on average, significantly (P < 0.001) more abundant in the phyllosphere of trees closest to the poultry operation (10% of relative abundance) than in trees 120 m into the orchard (1.7% relative abundance) or from any of the trees in control orchards (0.41% relative abundance). Poultry-associated microorganisms from a commercial operation transferred a short distance into an adjacent downwind almond orchard.

IMPORTANCE The movement of microorganisms, including foodborne pathogens, from animal operations into adjacent plant crop-growing environments is not well characterized. This study provides evidence that dust and bioaerosols moved from a commercial poultry operation a short distance downwind into an almond orchard and altered the microbiome recovered from the leaves. These data provide growers with information they can use to assess food safety risks on their property.

FOOTNOTES

    • Received 6 March 2020.
    • Accepted 14 May 2020.
    • Accepted manuscript posted online 22 May 2020.
  • Supplemental material is available online only.

  • [This article was published on 20 July 2020 with a standard copyright line (“© 2020 American Society for Microbiology. All Rights Reserved.”). The authors elected to pay for open access for the article after publication, necessitating replacement of the original copyright line with the one above, and this change was made on 5 August 2020.]

  • Copyright © 2020 Theofel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Microorganisms Move a Short Distance into an Almond Orchard from an Adjacent Upwind Poultry Operation
Christopher G. Theofel, Thomas R. Williams, Eduardo Gutierrez, Gordon R. Davidson, Michele Jay-Russell, Linda J. Harris
Applied and Environmental Microbiology Jul 2020, 86 (15) e00573-20; DOI: 10.1128/AEM.00573-20

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Microorganisms Move a Short Distance into an Almond Orchard from an Adjacent Upwind Poultry Operation
Christopher G. Theofel, Thomas R. Williams, Eduardo Gutierrez, Gordon R. Davidson, Michele Jay-Russell, Linda J. Harris
Applied and Environmental Microbiology Jul 2020, 86 (15) e00573-20; DOI: 10.1128/AEM.00573-20
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KEYWORDS

almond
bioaerosol
dust
microbiome
orchard
phyllosphere
poultry

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