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Environmental Microbiology | Spotlight

Modeling the Inactivation of Viruses from the Coronaviridae Family in Response to Temperature and Relative Humidity in Suspensions or on Surfaces

Laurent Guillier, Sandra Martin-Latil, Estelle Chaix, Anne Thébault, Nicole Pavio, Sophie Le Poder; on behalf of Covid-19 Emergency Collective Expert Appraisal Group, Christophe Batéjat, Fabrice Biot, Lionel Koch, Donald W. Schaffner, Moez Sanaa
Harold L. Drake, Editor
Laurent Guillier
aRisk Assessment Department, French Agency for Food, Environmental and Occupational Health and Safety, Maisons‐Alfort, France
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  • ORCID record for Laurent Guillier
Sandra Martin-Latil
bLaboratory for Food Safety, French Agency for Food, Environmental, and Occupational Health and Safety, University of Paris-EST, Maisons-Alfort, France
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Estelle Chaix
aRisk Assessment Department, French Agency for Food, Environmental and Occupational Health and Safety, Maisons‐Alfort, France
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Anne Thébault
aRisk Assessment Department, French Agency for Food, Environmental and Occupational Health and Safety, Maisons‐Alfort, France
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Nicole Pavio
cUMR Virologie 1161, ENVA, INRAE, Anses, Maisons-Alfort, France
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Sophie Le Poder
cUMR Virologie 1161, ENVA, INRAE, Anses, Maisons-Alfort, France
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Christophe Batéjat
dEnvironment and Infectious Risks Unit, Laboratory for Urgent Response to Biological Threats (CIBU), Institut Pasteur, Paris, France
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Fabrice Biot
eBacteriology Unit, French Armed Forces Biomedical Research Institute (IRBA), Brétigny-sur-Orge, France
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Lionel Koch
eBacteriology Unit, French Armed Forces Biomedical Research Institute (IRBA), Brétigny-sur-Orge, France
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Donald W. Schaffner
fDepartment of Food Science, Rutgers University, New Brunswick, New Jersey, USA
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Moez Sanaa
aRisk Assessment Department, French Agency for Food, Environmental and Occupational Health and Safety, Maisons‐Alfort, France
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Harold L. Drake
University of Bayreuth
Roles: Editor
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DOI: 10.1128/AEM.01244-20
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  • FIG 1
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    FIG 1

    Decimal reduction times of 10 coronaviruses according to temperature in suspension or on inert surfaces.

  • FIG 2
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    FIG 2

    Observed (points) and fitted (gray lines) log decimal reduction time values according to temperature for model 1 (A), model 2 (B), and model 3 (C). One thousand (1,000) bootstrap values of uncertainty characterization are shown. Estimates of model parameters are given in Table 2.

  • FIG 3
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    FIG 3

    (A) Observed inactivation rate values (gray points) according to temperature (°C) and relative humidity (%) and model 4 surface predictions. Scatter points of observed versus predicted D values (D in hours) for model 4 (B). The dashed line represents a perfect match between observations and predictions.

Tables

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  • TABLE 1

    Characteristics of the studies that explored inactivation of infectivity of coronavirusa

    TABLE 1
    • ↵a RH, relative humidity; VTM, viral transport medium; FCS, fetal calf serum; NPA, nasopharyngeal aspirate; TNS, throat and nasal swabs; MEM, minimal essential medium; PBS, phosphate-buffered saline; HRT, human rectal tumor; TCID50, 50% tissue culture infective dose(s); HDCS, human diploid cell strain; NLFK, Norden Laboratories feline kidney; –, data not specified. Symbols: *, not included (the limit of quantification reached for the first sample time); †, not included (not enough decrease was observed during experimentation).

  • TABLE 2

    Characteristics of the different models fitted to the 102 decimal reduction time data of coronaviruses according to temperature and relative humiditya

    TABLE 2
    • ↵a The temperature (Tref) was set at 4°C.

Additional Files

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      Appendix SA1

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      Appendix SA2

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    • Supplemental file 3 -

      Appendix SA3

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Modeling the Inactivation of Viruses from the Coronaviridae Family in Response to Temperature and Relative Humidity in Suspensions or on Surfaces
Laurent Guillier, Sandra Martin-Latil, Estelle Chaix, Anne Thébault, Nicole Pavio, Sophie Le Poder, Christophe Batéjat, Fabrice Biot, Lionel Koch, Donald W. Schaffner, Moez Sanaa on behalf of Covid-19 Emergency Collective Expert Appraisal Group
Applied and Environmental Microbiology Sep 2020, 86 (18) e01244-20; DOI: 10.1128/AEM.01244-20

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Modeling the Inactivation of Viruses from the Coronaviridae Family in Response to Temperature and Relative Humidity in Suspensions or on Surfaces
Laurent Guillier, Sandra Martin-Latil, Estelle Chaix, Anne Thébault, Nicole Pavio, Sophie Le Poder, Christophe Batéjat, Fabrice Biot, Lionel Koch, Donald W. Schaffner, Moez Sanaa on behalf of Covid-19 Emergency Collective Expert Appraisal Group
Applied and Environmental Microbiology Sep 2020, 86 (18) e01244-20; DOI: 10.1128/AEM.01244-20
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KEYWORDS

persistence
coronavirus
modeling
Fomites
SARS-CoV-2

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