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Genetics and Molecular Biology

Throat Microbial Community Structure and Functional Changes in Postsurgery Laryngeal Carcinoma Patients

Chi-Yao Hsueh, Hongli Gong, Ning Cong, Ji Sun, Hui-Ching Lau, Yang Guo, Qiang Huang, Xiaohui Yuan, Ming Zhang, Lei Tao, Liang Zhou
Harold L. Drake, Editor
Chi-Yao Hsueh
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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  • ORCID record for Chi-Yao Hsueh
Hongli Gong
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Ning Cong
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Ji Sun
bDepartment of Pathology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Hui-Ching Lau
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Yang Guo
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Qiang Huang
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Xiaohui Yuan
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Ming Zhang
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Lei Tao
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Liang Zhou
aDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China
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Harold L. Drake
University of Bayreuth
Roles: Editor
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DOI: 10.1128/AEM.01849-20
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ABSTRACT

The microbial community structure in the throat and its shift after laryngectomy in laryngeal squamous cell carcinoma (LSCC) patients were investigated. Thirty swab samples taken prior to laryngectomy (SLC), 18 samples 1 week after laryngectomy (SLCA1w), and 30 samples 24 weeks after laryngectomy (SLCA24w) from 30 LSCC patients were examined. Microbial diversity was profiled through sequencing the V3-V4 variable region of the 16S rRNA gene. Quantitative real-time PCR (qPCR) was used to validate the 16S rRNA sequence data for the V3-V4 region. The community structure and function of throat microbiota were assessed by PICRUSt (phylogenetic investigation of communities by reconstruction of unobserved states) analysis. Both alpha and beta diversity results showed significant differences in the throat microbiota of LSCC patients before and after laryngectomy (P < 0.05). The drinking index of the SLC group was positively associated with the genus abundance of Prevotella (P < 0.05). The SLCA1w group had lower abundances of Fusobacterium, Leptotrichia, Lachnoanaerobaculum, and Veillonella than the SLC group (P < 0.05). The SLCA24w group had higher abundances of Streptococcus and Leptotrichia as well as lower abundances of Fusobacterium and Alloprevotella than the SLC group (P < 0.05). The throat microbiomes of the SLC group could be implicated in human cancer signaling pathways, as evidenced by PICRUSt analysis (P < 0.05). Our study clarifies alterations in throat microbial community structure and function in LSCC patients during the perioperative period and postoperative recovery period.

IMPORTANCE Laryngeal squamous cell carcinoma greatly impacts patients’ lives, and noninvasive means of prognostic assessment are valuable in determining the effectiveness of laryngectomy. We set out to study the microbial structure changes in the throat before and after laryngectomy and found the gene functions of several throat bacteria to be associated with human cancer signaling pathways. Our findings may offer insights into the disease management of patients with laryngeal squamous cell carcinoma. We hope to provide a means of using molecular mechanisms to improve the prognosis of laryngeal cancer treatment and to facilitate relevant research.

FOOTNOTES

    • Received 30 July 2020.
    • Accepted 14 September 2020.
    • Accepted manuscript posted online 2 October 2020.
  • Supplemental material is available online only.

  • Copyright © 2020 American Society for Microbiology.

All Rights Reserved.

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Throat Microbial Community Structure and Functional Changes in Postsurgery Laryngeal Carcinoma Patients
Chi-Yao Hsueh, Hongli Gong, Ning Cong, Ji Sun, Hui-Ching Lau, Yang Guo, Qiang Huang, Xiaohui Yuan, Ming Zhang, Lei Tao, Liang Zhou
Applied and Environmental Microbiology Nov 2020, 86 (24) e01849-20; DOI: 10.1128/AEM.01849-20

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Throat Microbial Community Structure and Functional Changes in Postsurgery Laryngeal Carcinoma Patients
Chi-Yao Hsueh, Hongli Gong, Ning Cong, Ji Sun, Hui-Ching Lau, Yang Guo, Qiang Huang, Xiaohui Yuan, Ming Zhang, Lei Tao, Liang Zhou
Applied and Environmental Microbiology Nov 2020, 86 (24) e01849-20; DOI: 10.1128/AEM.01849-20
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KEYWORDS

laryngeal squamous cell carcinoma
laryngectomy
microbiota

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