RT Journal Article SR Electronic T1 Fungal Endophytic Communities in Grapevines (Vitis vinifera L.) Respond to Crop Management JF Applied and Environmental Microbiology JO Appl. Environ. Microbiol. FD American Society for Microbiology SP 4308 OP 4317 DO 10.1128/AEM.07655-11 VO 78 IS 12 A1 Pancher, Michael A1 Ceol, Marco A1 Corneo, Paola Elisa A1 Longa, Claudia Maria Oliveira A1 Yousaf, Sohail A1 Pertot, Ilaria A1 Campisano, Andrea YR 2012 UL http://aem.asm.org/content/78/12/4308.abstract AB We studied the distribution of fungal endophytes of grapevine (Vitis vinifera L.) plants in a subalpine area of northern Italy, where viticulture is of high economic relevance. We adopted both cultivation-based and cultivation-independent approaches to address how various anthropic and nonanthropic factors shape microbial communities. Grapevine stems were harvested from several locations considering organic and integrated pest management (IPM) and from the cultivars Merlot and Chardonnay. Cultivable fungi were isolated and identified by internal-transcribed-spacer sequence analysis, using a novel colony-PCR method, to amplify DNA from fungal specimens. The composition of fungal communities was assessed using a cultivation-independent approach, automated ribosomal intergenic spacer analysis (ARISA). Multivariate statistical analysis of both culture-dependent and culture-independent data sets was convergent and indicated that fungal endophytic communities in grapevines from organically managed farms were different from those from farms utilizing IPM. Fungal communities in plants of cv. Merlot and cv. Chardonnay overlapped when analyzed using culture-dependent approaches but could be partially resolved using ARISA fingerprinting.