RT Journal Article
SR Electronic
T1 Identification of Genes Involved in Cytochrome <em>c</em> Biogenesis in <em>Shewanella oneidensis</em>, Using a Modified <em>mariner</em> Transposon
JF Applied and Environmental Microbiology
JO Appl. Environ. Microbiol.
FD American Society for Microbiology
SP 4935
OP 4937
DO 10.1128/AEM.71.8.4935-4937.2005
VO 71
IS 8
A1 Bouhenni, R.
A1 Gehrke, A.
A1 Saffarini, D.
YR 2005
UL http://aem.asm.org/content/71/8/4935.abstract
AB A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome c maturation genes ccmC and ccmF1, respectively, were analyzed. Both mutants were deficient in anaerobic respiration and cytochrome c production.