Table 1.

Calculated yields for D. dehalogenans grown in the presence of various terminal electron acceptors

Electron-donating half-reactionaElectron-accepting half-reactionaYield (g/mol)b
YX/S(pyruvate)cYX/acetateYX/reduced acceptor
dPyruvate + H2O → acetate + CO2 + 2e + 2H+ (1 ATP)Pyruvate + 2e + 2H+→ lactate6.1 ± 0.513.4 ± 1.613.0 ± 1.8
12.2 ± 1.0
ePyruvate + H2O → acetate + CO2 + 2e + 2H+ (1 ATP)3-Cl-4-OHPA + 2e + 2H+ → 4-OHPA + HCl (1 ATP)25.4 ± 2.624.2 ± 1.624.6 ± 1.9
fPyruvate + H2O → acetate + CO2 + 2e + 2H+ (1 ATP)Fumarate + 2e + 2H+ → succinate (1 ATP)24.3 ± 2.525.9 ± 2.423.4 ± 2.0
g4Pyruvate + 4H2 → 4acetate + 4CO2 + 8e + 8H+ (4 ATP)Nitrate + 8e + 8H+ → NH4+(1 ATP)22.0 ± 0.923.8 ± 0.821.1 ± 2.9i
h3Pyruvate + 3H2O → 3acetate + 3CO2 + 6e + 6H+ (3 ATP)Sulfite + 6e + 6H+ → S2− (1 ATP)21.2 ± 3.723.3 ± 2.623.6 ± 2.1
  • a Assumed ATP formation.

  • b Standard deviations calculated for the slope in the graph of dry weight biomass versus the corresponding parameter. The values were determined for duplicate experiments.

  • c Pyruvate serves as both electron donor and acceptor. The second YX/Svalue was calculated based on the assumption that 1 mol of pyruvate is reduced per mol oxidized.

  • d 16.4 mol of pyruvate → 7.5 mol of acetate + 7.7 mol of lactate + 100 g of biomass.

  • e 3.9 mol of pyruvate + 4.1 mol of 3-Cl-4-OHPA → 4.1 mol of acetate + 4.07 mol of 4-OHPA + 100 g of biomass.

  • f 4.1 mol of pyruvate + 4.4 mol of fumarate → 3.9 mol of acetate + 4.3 mol of succinate + 100 g of biomass.

  • g 4.55 mol of pyruvate + 1.2 mol of nitrate → 4.2 mol of pyruvate + 1.2 mol of ammonia + 100 g of biomass.

  • h 4.7 mol of pyruvate + 1.4 mol of sulfite → 4.3 mol of acetate + 1.4 mol of sulfide + 100 g of biomass.

  • i YX/reduced acceptor was calculated based on nitrate because all nitrate utilized was completely reduced to ammonia.