Table 1.

Effects of α- and β-pinenes on CH4oxidation by methanotrophs incubated with and without Cu

SupplementAmt of CH4 oxidized (μmol flask−1)a
Methylosinus trichosporiumOB3bbMethylobacter luteus with Cuc
Without CuWith Cu
None (control)300 ± 6278 ± 20133 ± 11
(−)-α-Pinene73 ± 7 (76)45 ± 21 (84)11 ± 15 (92)
(+)-α-Pinene187 ± 7 (38)26 ± 8 (91)21 ± 11 (84)
(−)-β-Pinene287 ± 13 (4)158 ± 15 (43)56 ± 4 (58)
  • a The values are means ± standard errors of the means determined from triplicate experiments. The values in parentheses are percentages of inhibition compared with the control.

  • b Measured after 2 days of incubation with an initial CH4 content of 20% (vol/vol) and a cell OD600 of 0.095.

  • c Measured after 1 day of incubation with an initial CH4 content of 10% (vol/vol) and a cell OD600 of 0.095.