Table 1.

Purification of LipP from E. coli C600 harboring pPL2-1

Purification methodTotal protein (mg)Total activity (Ua)Sp act (U/mg)Purifi- cation (fold)Yield (%)
Cell extract1805,200291100
DEAE-Cellulofine5.31,3002508.625
Gigapite0.88601,1003817
  • a Activity was measured by determination of 2,3-dimercaptopropan-1-ol formed from 2,3-dimercaptopropan-1-ol tributyl ester with 5,5′-dithio-bis(2-nitrobenzoic acid). The definition of unit is given in the text.