Table 2.

Theoretical alignment of sequences of amplimers 63f and 1387r with database sequences of 16S rRNA genes from species not tested by PCR

StrainWoese groupingSequence vsa:
63f1387r
CAGGCCTAACACATGCA—AGTC CGGAACATGTGWGGCGGG
Fibrobacter intestinales Fibrobacterspp. AGT G T CT T A T ACATGCA—AGTC GCCTTGTACACACCGCCC
Chlorobium limicola Green non-sulfur C GT GCCTAACACATGCA—AGTC GCCTTGTACACACCGCCC
Chloroflexus aurantiacus Green non-sulfur C GT GCCTAA TG CATGCA—AGTC GCCTTGTACACACCGCCC
Herpetosiphon aurantiacus Green non-sulfur C GT GCCTAA TG CATGCA—AGTC GCCTTGTACACACCGCCC
Thermomicrobium roseum Green non-sulfur C GT GCCTAA TG CATGCA—AGTC GCCTTGTACACACCGCCC
Deinococcus radiodurans Radioresistant micrococci and relatives C GT GC T TAA G ACATGCA—AGTC GCCTTGTACACACCGCCC
Thermus aquaticus Radioresistant micrococci and relatives C GT GCCTAA G ACATGCA—AGTC GCCTTGTACACACCGCCC
Thermus ruber Radioresistant micrococci and relatives T A T GCCTAA G ACATGCA—AGTC GCCTTGTACACACCGCCC
Fervidobacterium nodosum Thermotogales C GT G C CTAACACATGCA—AGTC GCCTTGTACACACCGCCC
Thermotoga elfii Thermotogales C GT G CT TAACACATGCA—AGTC G GT TTGCACACACCGCCC
Thermotoga maritima Thermotogales C GT G C CTAACACATGCA—AGTC GCCTTGTACACACCGCCC
  • a Nucleotides underlined and in boldface differ from corresponding nucleotides in amplimer.