TABLE 3.

Results of control hybridizations for CFB-specific 16S rDNA-targeted oligonucleotide probesa

SpeciesCatalogue no.PhylogenyHybridization with:
CFB560CFB562CFB376CF319a/b
CFBs
    Cytophaga fermentansNCIMB 2218 Bacteroides +++
    Bacteroides distasonisATCC 8503 Bacteroides +++
    Bacteroides fragilisATCC 25285 Bacteroides ++
    Bacteroides eggerthiiNCTC 11185 Bacteroides ++
    Microscilla aggregansNCIMB 1418 Cytophaga ++
    Chryseobacterium indoltheticumNCIMB 2220 Cytophaga ++
    Flavobacterium aquatileNCIMB 8694 Cytophaga ++
    Flexibacter maritimusNCIMB 2154 Cytophaga ++
    Cytophaga uliginosaNCIMB 1863 Cytophaga ++
    Cytophaga laterculaNCIMB 1399 Cytophaga ++
    Cytophaga marinoflavaATCC 19326 Cytophaga ++
    Pedobacter heparinusNCIMB 9290 Sphingobacterium ++
    Saprospira grandisNCIMB 1363 Lewinella ++++
    Microscilla arenariaNCIMB 1413 Persicobacter +++
    Microscilla sericeaNCIMB 1403 Cytophaga aurantiaca ++++
Non-CFBs
    Agrobacterium tumefaciensPutative isolateα-Proteobacteria
    Burkholderia cepaciaNCTC 10661β-Proteobacteria
    Acinetobacter calcoaceticusNCIMB 8250γ-Proteobacteria
    Proteus vulgarisNCTC 6213γ-Proteobacteria
    Pseudomonas fragiNCTC 10689γ-Proteobacteria
    Pseudomonas aeruginosaPutative isolateγ-Proteobacteria
    Myxococcus xanthusNCIMB 9412δ-Proteobacteria
    Synechococcus sp.PCC 7942 Cyanobacteria
    Synechocystis sp.PCC 6803 Cyanobacteria
    Nostoc sp. macPCC 8009 Cyanobacteria
    Nostoc sp.PCC 7524 Cyanobacteria
    Anabaena sp.PCC 7126 Cyanobacteria
    Bacillus megateriumATCC 12872Gram positive
    Bacillus cereusNCTC 7464Gram positive
    Bacillus subtilisNCTC 3610Gram positive
    Staphylococcus aureusNCTC 6571Gram positive
    Brevibacter acetylicumPutative isolateGram positive
    Arthrobacter atrocyaneusPutative isolateGram positive
    Clone 83.8% related to Verrucomicrobiales clone CO19 Verrucomicrobiales
    Clone 87.8% related to Pirellula marina Planctomyces
  • a Probes CFB560, CFB562, and CFB376 (this study) and probe CF319a/b (32). PCR-amplified 16S rDNAs from target and nontarget bacteria were fixed onto nylon membrane and hybridized against probes under optimized conditions. The phylogeny of test organisms stated here is as listed in the RDP (release 8.0) small-subunit phylogentic trees.