TABLE 1

Kinetic parameters of BpUrease

Substrate and enzymeSp act (U · mg−1)Km (mM)Vmax (μmol · mg−1 · min−1)kcat (s−1)kcat/Km (s−1 · M−1)
Urethane
    BpUrease (Ni2+)23.1 ± 0.41,018 ± 58118 ± 5433 ± 22425 ± 19
    BpUrease (Fe3+)2.5 ± 0.2958 ± 5215.2 ± 1.156 ± 358 ± 3.9
Urea
    BpUrease (Ni2+)603 ± 1323.7 ± 1.5769 ± 392,820 ± 135(1.2 ± 0.1)E + 05
    BpUrease (Fe3+)54.6 ± 2.128.2 ± 1.773.5 ± 5.3270 ± 149,557 ± 478