TABLE 2.

Primers used in this study

PrimeraPrimer sequence (5′-3′)Source or reference
S-D-Bact-0011-a-S-17AGA GTT TGA T (C/T) (A/C) TGG CTC AG16
S-D-Bact-0124-a-S-27GGA CGG GTG AGT AAC ACG18
S-D-Lab-0158-a-S-20TGG AAA CAG (A/G) TG CTA ATA CC11
S-G-Lab-0159-a-S-20GGA AAC AG (A/G) TGC TAA TAC CGThis study
S-*-Univ-0515-a-A-24ATC GTA TTA CCG CGG CTG CTG GCA18
S-G-Lab-0677-a-A-17CAC CGC TAC ACA TGG AGThis study
S-G-Lacb-0722-a-A-25(C/T) CA CCG CTA CAC ATG (A/G) AG TTC CAC T39
GC-clampCGC CGG GGG CGC GCC CCG GGC GGG GCG GGG GCA CGG GGG G29
T7TAA TAC GAC TCA CTA TAG GPromega
Sp6GAT TTA GGT GAC ACT ATA GPromega
  • a Apart from the GC clamp and primers T7 and Spb, the nomenclature is according to Alm et al. (2).