TABLE 3.

Alignment of primer S-G-Lab-0677-a-A-17 with target sequences of 16S rDNA (region 677 to 693 of E. coli numbering) from Lactobacillus and other species

Primer or species (no. of species found)aSequence
S-G-Lab-0677-a-A-17 reverse primer5′-C ACC GCT ACA CAT GGA G-3′
All Lactobacillus spp. subgroupsb (94)GTA AGG TGG CGA TGT GTA CCT CAA G
All Leuconostoc spp. (18)GTA AGG TGG CGA TGT GTA CCT CAA G
All Weissella spp. (16)GTA AGG TGG CGA TGT GTA CCT CAA G
All Pediococcus spp. (12)GTA AAG TGG CGA TGT GTA CCT CAA G
Bacteroides fragilisGTA AAG TGG CGA TGT GGT GCT TAA G
Bacteroides vulgatusGTA AAG TGG CGA TGT GGT GCT TAA G
Bifidobacterium bifidumGTA AGG TGG CAA TGT GGC CCT TAA G
Clostridium coccoidesGTA AAG TGG CGA TGT GAT CCT TAA G
Clostridium perfringensGTA AAG TGG CGA TGT GAT CCT TAA G
Clostridium ramosumGTA AAG TGG CGA TGT GTA CCT TAA G
Enterococcus faecalisGTA AAG TGG CGA TGT GTA CCT TAA G
Eubacterium biformeGTA AAA TGG CGA TGT GTA CCT CAA G
Eubacterium rectaleGTA AAG TGG CGA TGT GAT CCT TAA G
Lactococcus lactisGTA AAG TGG CGA TGT GTA CCT TAA G
Staphylococcus aureusGTA AAG TGG CGA TGT GTA CCT TAA G
Streptococcus thermophilusGTA AAG TGG CGA TGT GTA CCT TAA G
Vagococcus fluvialisGTA AAG TGG CGA TGT GTA CCT TAA G
  • a The number of species found in the database is indicated in parentheses. The mismatches with the primer sequence are indicated in boldface and are underlined.

  • b Includes all Lactobacillus species in GenBank, except Lactobacillus maltaromicus, L. catenaformis, and L. vitulinus.