TABLE 4.

Phylogenetic affinity of sequences co-migrating with DGGE bands in Fig. 2a

BandSampleAccession no. of cloned sequenceClosest sequenceb (accession no.)Groupc% SimilarityNo. of clones
A96BD125 BP (not submitted)Sva0996 (AJ241005)High G+C, gram positive960
B96BDAF355053Clone SAR307 (U20798)GNSB972
C97AAF355044Nitrospina gracilis (L35504)δ911
D96AAF353233Clone OM38 (U70679)α, SAR116981
E95CAF355042Nitrospina gracilis (L35504)δ892
F95AAF353227Clone SAR407 (U75253)α, SAR11984
G96ADAF353212Clone SAR203 (U75255)α, SAR11971
H96AAF353208Clone SAR407 (U75253)α, SAR119823
H96AAF353234Clone NAC1-19 (AF245628)α, Roseo971
I96BAF354595Fundibacter jadensis (AJ001150)γ881
I96BDAF355041Clone NAC60-12 (AF245652)δ981
I96BAF353224Clone SAR407 (U75253)α, SAR11965
I96AAF353235Clone NAC11-3 (AF245632)α, Roseo998
I95BAF353214Clone ZD0410 (AJ400351)α, SAR11997
J96ADAF354601Pseudoalteromonas atlantica (AF173963)γ992
K96ADAF355040Clone NAC60-12 (AF245652)δ995
L96BAF353229Clone OM155 (U70686)α, SAR11952
M96BAF353242No matchγ3
M95BAF353241Oceanospirillum sp. (AJ302699)γ901
N95AAF354598Alteromonas macleodii (Y18228)γ9910
O95BAF353239Oceanospirillum sp. (AJ302699)γ967
P96ADAF354604Alcanivorax borkumensis (Y12579)γ962
P96BAF354597Pseudoalteromonas atlantica (AF173963)γ981
P96AAF354611Clone ZD0424 (AJ400355)γ972
P95BAF354609Fundibacter jadensis (AJ001150)γ981
Q96BAF354596Pseudomonas fluorescens (AF094726)γ911
R96ADAF354607Thiomicrospira sp. (AJ237758)γ901
S196AAF354610Fundibacter jadensis (AJ001150)γ983
S196BDAF354605Alcanivorax borkumensis (Y12579)γ942
S295DAF354615Clone OM241 (U70702)γ931
S396BAF354616Clone ZD0403 (AJ400347)Cyto911
T97AAF354617Strain agg58 (L10946)Cyto851
U196BDAF354606Clone ZD0405 (AJ400348)γ991
U297AAF354613Clone OCS5 (AF001651)γ, SAR86991
V196BDAY08221Clone DEV005 (AJ401105)Verr881
V296BAF354621Polaribacter irgensii (M61002)CFB971
V297AAF354618Clone OM271 (U70708)Cyto931
V397AAF355051Clone ZD0403 (AJ400347)Cyto911
W97AAF354620Polaribacter irgensii (M61002)CFB952
  • a With the exception of band A, which was identified by sequencing DNA contained in the band, identifications were made by comparing the mobility of fragments amplified from cloned inserts with the mobility of fragments amplified from samples. Where more than one clone contained an insert of a given phylotype that matched a band, the accession number given is for the clone with the longest sequence with the greatest BLAST similarity value to the database sequence and the number of clones matching that phylotype is given. Twenty-one clones contained sequences that could not be assigned to bands in Fig. 2.

  • b As determined by BLAST.

  • c Abbreviations are as defined in Table 2, footnote b.