TABLE 4.

Phylogenetic affinity of sequences co-migrating with DGGE bands in Fig. 2a

BandSampleAccession no. of cloned sequenceClosest sequenceb (accession no.)Groupc% SimilarityNo. of clones
A96BD125 BP (not submitted)Sva0996 (AJ241005 )High G+C, gram positive960
B96BD AF355053 Clone SAR307 (U20798 )GNSB972
C97A AF355044 Nitrospina gracilis (L35504 )δ911
D96A AF353233 Clone OM38 (U70679 )α, SAR116981
E95C AF355042 Nitrospina gracilis (L35504 )δ892
F95A AF353227 Clone SAR407 (U75253 )α, SAR11984
G96AD AF353212 Clone SAR203 (U75255 )α, SAR11971
H96A AF353208 Clone SAR407 (U75253 )α, SAR119823
H96A AF353234 Clone NAC1-19 (AF245628 )α, Roseo971
I96B AF354595 Fundibacter jadensis (AJ001150 )γ881
I96BD AF355041 Clone NAC60-12 (AF245652 )δ981
I96B AF353224 Clone SAR407 (U75253 )α, SAR11965
I96A AF353235 Clone NAC11-3 (AF245632 )α, Roseo998
I95B AF353214 Clone ZD0410 (AJ400351 )α, SAR11997
J96AD AF354601 Pseudoalteromonas atlantica (AF173963 )γ992
K96AD AF355040 Clone NAC60-12 (AF245652 )δ995
L96B AF353229 Clone OM155 (U70686 )α, SAR11952
M96B AF353242 No matchγ3
M95B AF353241 Oceanospirillum sp. (AJ302699 )γ901
N95A AF354598 Alteromonas macleodii (Y18228 )γ9910
O95B AF353239 Oceanospirillum sp. (AJ302699 )γ967
P96AD AF354604 Alcanivorax borkumensis (Y12579 )γ962
P96B AF354597 Pseudoalteromonas atlantica (AF173963 )γ981
P96A AF354611 Clone ZD0424 (AJ400355 )γ972
P95B AF354609 Fundibacter jadensis (AJ001150 )γ981
Q96B AF354596 Pseudomonas fluorescens (AF094726 )γ911
R96AD AF354607 Thiomicrospira sp. (AJ237758 )γ901
S196A AF354610 Fundibacter jadensis (AJ001150 )γ983
S196BD AF354605 Alcanivorax borkumensis (Y12579 )γ942
S295D AF354615 Clone OM241 (U70702 )γ931
S396B AF354616 Clone ZD0403 (AJ400347 )Cyto911
T97A AF354617 Strain agg58 (L10946 )Cyto851
U196BD AF354606 Clone ZD0405 (AJ400348 )γ991
U297A AF354613 Clone OCS5 (AF001651 )γ, SAR86991
V196BD AY08221 Clone DEV005 (AJ401105 )Verr881
V296B AF354621 Polaribacter irgensii (M61002 )CFB971
V297A AF354618 Clone OM271 (U70708 )Cyto931
V397A AF355051 Clone ZD0403 (AJ400347 )Cyto911
W97A AF354620 Polaribacter irgensii (M61002 )CFB952
  • a With the exception of band A, which was identified by sequencing DNA contained in the band, identifications were made by comparing the mobility of fragments amplified from cloned inserts with the mobility of fragments amplified from samples. Where more than one clone contained an insert of a given phylotype that matched a band, the accession number given is for the clone with the longest sequence with the greatest BLAST similarity value to the database sequence and the number of clones matching that phylotype is given. Twenty-one clones contained sequences that could not be assigned to bands in Fig. 2.

  • b As determined by BLAST.

  • c Abbreviations are as defined in Table 2, footnote b.