TABLE 2.

Osmotically induced ectoine biosynthesis in B. pasteurii (DSM 33T)

MediumaEctoine (mmol per g [dry weight])b
SMM (680 mosmol per kg of water)NDc
SMM with 0.4 M NaCl (1,400 mosmol per kg of water)0.40 ± 0.020
SMM with 0.4 M KCl (1,400 mosmol per kg of water)0.59 ± 0.034
SMM with 0.68 M sucrose (1,400 mosmol per kg of water)0.59 ± 0.070
SMM with 0.68 M lactose (1,400 mosmol per kg of water)0.36 ± 0.067
SMM with 1.1 M glycerol (1,400 mosmol per kg of water)ND
SMM with 0.4 M NaCl + 1 mM glycine betaine (1,400 mosmol per kg of water)ND
SMM with 0.68 M sucrose + 1 mM glycine betaine (1,400 mosmol per kg of water)ND
  • a Cultures of B. pasteurii (DSM 33T) were grown in SMM (2% urea) with the indicated ionic and nonionic osmolytes. Ectoine was extracted from the freeze-dried cell pellet and was measured by HPLC analysis.

  • b The data shown are means of two independently grown cultures, and for each of these cultures the ectoine content was determined twice.

  • c ND, no ectoine was detectable.