TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
E. coli
  HB101 supE44 hsdS20 (rB mB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1Takara, Osaka, Japan
  JM109 recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB)
F′ [traD36 proAB+lacIqlacZΔM15]Takara, Osaka, Japan
Rhizobium sp.
  AC100Wild typeThis study
  AC101CarThis study
  AC1012Car Strr RifrThis study
Plasmids
 pRK2013Kmrtra+; triparental mating helper, ColE1 replication 13
 pBI101Kmr; broad-host-range cloning vectorClontech
 pBluescript II SK(+)Ampr; sequencing vectorStratagene
 pCEH-B23-kb BamHI fragment from pAC200 cloned into pBI101This study
 pCEH-Sa6.2-kb SalI fragment from pAC200 cloned into pBI101This study
 pCEH-Sm7.2-kb SmaI fragment from pAC200 cloned into pBI101This study
 pCEH-EVB3.2-kb EcoRV-BamHI fragment from pCEH-B subcloned into pBI101This study
 pCEH-EVSc2.7-kb EcoRV-SacII fragment from pCEH-B subcloned into pBI101This study