TABLE 1

Gene annotation for glc and ben loci of Pseudomonas putida CSV86

Locus (contig no.)Gene (no. of bp/amino acids)Annotated proteinaGenBank accession no.Closest homologous protein, isolate, accession no.Percent identity
glc (84)gbpc (1,266/421)GBP (PBP)EKX85295GBP, Pseudomonas putida CBF10-2, OAI9437299
UgpB, Ochrobactrum anthropi, 4R2B_Ab48
MBP, Thermus thermophiles, 2B3B_Ab30
glcF (909/302)GlcF (permease)EKX85296GlcF, Pseudomonas putida CBF10-2, OAI9437399
LplB, Sphingomonas sp. S7-754, 4TQU_Mb22
glcG (834/277)GlcG (permease)EKX85297GlcG, Pseudomonas putida CBF10-2, OAI94374100
MalG, Escherichia coli K-12, 2R6G_Gb30
glcKc (1,155/384)GlcK (ATPase)EKX85298GlcK, Pseudomonas putida CBF10-2, OAI94375100
UgpC, Sphingomonas sp. S7-754, 4TQU_Sb49
MalK, Thermus thermophiles, 1G29_1b46
oprB (1,308/435)OprB (OMP)EKX85299OprB, Pseudomonas putida CBF10-2, OAI94376100
OprB, Pseudomonas putida BIRD-1, 4GEY_Ab87
ben (175)benF (1,236/411)BenF (OMP)EKX82693OprD, Pseudomonas putida CBF10-2, OAI9266199
OpdK, Pseudomonas aeruginosa, 2QTK_Ab49
benE (1,203/400)BenE (benzoate-H+ symporter family)EKX82694BenE, Pseudomonas putida CBF10-2, OAI9266299
BenE, Pseudomonas thermotolerans, WP_01793904381
catA (926/308)Catechol 1,2-dioxygenaseEKX82695CatA, Pseudomonas putida CBF10-2, OAI9266399
CatA, Pseudomonas arvilla C-1, 2AZQ_Ab79
benK (1,338/445)BenK (MFS)EKX82696BenK, Pseudomonas putida CBF10-2, OAI9270699
XylE (MFS), Escherichia coli K-12, 4QIQ_Ab27
  • a PBP, periplasmic substrate binding protein; OMP, outer membrane porin; MFS, major facilitator superfamily protein.

  • b Structurally/functionally characterized homolog (PDB accession number).

  • c Genes are expressed in E. coli and characterized functionally.