TABLE 1

Purification of the NADH-dependent [FeFe]-hydrogenase Hyd1ABC

FractionProtein (mg)Activity H2 → MVox (U · mg−1)aPurification (fold)Yield (%)
SpecificTotal
Cell extract67016.811,2001100
HisTrap HP4.06552,62038.923.4
UNO-Q10.433,3401,43019912.7
  • a Activity was determined at 37°C, pH 7.5; 1 unit of activity (U) equals 2 μmol of electrons transferred per minute. MVox, oxidized methyl viologen.