TABLE 4

List of primers used for construction of pETDuet-1 SwHydABC, pCDFDuet-1 SwHydEFG, and pCDFDuet-1 SwFd

Primers (F, R)aFunction
CGCGGATCCGATGATGGATTATAAGGAGATAATTGCCCAG, TTGGCGCGCCCTATAAGAATTTTTTATTCTTGGCATGGAmplify SWOL_RS05165, SWOL_RS05170, SWOL_RS05175, addition of restriction cut sites for BamHI/AscI
TAACCATATGCAGGATACTCCCAAAGCTA, TAACCTCGAGTTAAAGAATTGCTCGGACCCTGAmplify SWOL_RS01625, addition of restriction cut sites for NdeI/XhoI
TTCACCATGGAAGCGATATCAGTTAACC, ACATGGATCCTTAAACCTGCTTCAAAGGAmplify SWOL_RS05180, SWOL_RS05185, SWOL_RS05190, addition of restriction cut sites for NcoI/BamHI
CTTAGGATCCGATGTCTTACATCATC, AGTGATAAGCTTTTAGTCGTCCGAmplify SWOL_RS010890, addition of restriction cut sites for BamHI/HindIII
  • a Underlined regions indicate restriction enzyme cut sites. F, forward; R, reverse.