Table 1.

Phylogenetic affiliations based on SSU rDNA genes of members of a Hawaiian rainforest soil bacterial communitya

PhylotypeRelative abundance (%)bPhylogenetic affiliationMost closely related organism in RDP database% Similarity to most closely related organism
TaxonRDP subdivision
35% G+C fraction
 HRS-16.8Clostridia and their relatives[Clostridium tetani]cClostridium novyi subgroup80.6
 HRS-26.8Clostridia and their relatives[Clostridium tetani]Clostridium novyi subgroup76.1
 HRS-36.8Clostridia and their relativesClostridium fallaxClostridium perfringensassemblage92.9
 HRS-46.8Clostridia and their relativesClostridium fallaxClostridium perfringensassemblage94.0
 HRS-65.1Clostridia and their relativesClostridium puniceumClostridium butyricumsubgroup95.3
 HRS-71.7Clostridia and their relativesClostridium butyricumClostridium butyricumsubgroup94.7
 HRS-81.7Clostridia and their relativesClostridium puniceumClostridium butyricumsubgroup98.0
 HRS-101.7Clostridia and their relativesClostridium beijerinckiiClostridium butyricumsubgroup98.5
 HRS-211.7Clostridia and their relativesClostridium puniceumClostridium butyricumsubgroup98.5
 HRS-221.7Clostridia and their relatives[Clostridium tetanomorphum]Clostridium butyricum subgroup80.0
63% G+C fraction
 HRS-1213.2Alpha purple bacteria[Rhodopseudomonas viridis]Rhizobium-Agrobacterium group82.6
 HRS-1310.5Gamma purple bacteriaPseudomonas syringaePseudomonas subgroup97.9
 HRS-167.9Alpha purple bacteriaAzospirillum lipoferumRhodospirillum rubrum assemblage88.0
 HRS-171.3Alpha purple bacteriaZoogloea ramigeraBrucella assemblage90.4
 HRS-181.3FibrobacterphylumEnvironmental strain MC 103Acidobacteriumsubdivision93.2
 HRS-191.3Bacillus-Lactobacillussubdivision[Bacillus flavothermus]Bacillus megaterium group80.3
 HRS-201.3Delta purple bacteriaEnvironmental strain FIE 20Myxobacteriumgroup92.9
  • a Only unambiguously aligned regions were used in the analysis.

  • b Relative abundance of clones belonging to a phylotype, calculated by dividing the number of clones belonging to the phylotype by the total number of clones analyzed.

  • c Brackets indicate that the taxonomic assignment of the closest relative is uncertain (level of similarity, less than 85%).