Table 1.

Association of IS1071 with catabolic genes or operons in independent isolates from various sourcesa

Genetic element(s)bCatabolic genotypecCompound(s) degradedSize (kb)No. of IS1071copiesdHost(s)SourceReference(s)
Tn5271, pBRC60cbaABCChlorobenzoates17.12eAlcaligenessp. strain BR60Hyde Park landfill, Lewiston, N.Y.28, 30, 32
pUO1dehH2Chloroacetate8.62eMoraxellasp. strain BIndustrial wastewater, Japan23, 24, 51
pTDN1tdnQTA1A2BRAromatic amines302ePseudomonas putida UCC22, a strain mt-2 (TOL) derivativeWales14
pOPH1phto-Phthalate702Comamonas acidovorans UCC61Sewage sludge, Wales9
IS1071::IS1471, pIJB1tfd2,4-Dichlorophenoxyacetate4.31e,fBurkholderia cepacia 2aSoil, United Kingdom54
pPOBpobABCarboxydiphenyl ethersNDg1e,hPseudomonas pseudoalcaligenes POB310Soil and activated sludge, Germany7
pPSB, pTSApsbACp-SulfobenzoateND2e,iComamonas testosteroni T-2 and PSB-4Soils, Switzerland21, 22
pCPE3cbaABCChlorobenzoates16.22eAlcaligenessp. strain CPE3PCB-contaminated soils, Italy11; this study
  • a The data have been summarized previously (53).

  • b A transposon designation is given where known; otherwise the plasmid designation is given.

  • c cbaABC, chlorobenzoate 3,4-(4,5)-dioxygenase, reductase, and dehydrogenase genes; dehH2, chloroacetate dehalogenase gene; tdnQTA1A2BR, aniline and toluidine catabolic genes; pht, phthalate catabolic genes; tfd, 2,4-dichlorophenoxyacetate catabolic genes; pobAB, p-phenoxybenzoate catabolic genes;psbAC, p-sulfobenzoate catabolic genes.

  • d Elements with two copies have direct repeats of IS1071 flanking the catabolic genes.

  • e Complete or partial IS1071sequences are known.

  • f The IS1071 tnpA gene on pIJB1 is interrupted by a class I insertion sequence, IS1471 (1.1 kb), which is inserted at nucleotide 864 of IS1071.

  • g ND, not determined.

  • h The one copy of IS1071 is located 356 bp 5′ to the start codon of pobA.

  • i Two copies of IS1071 are linked to mobilization of the psbAC genes from pPSB to the chromosome of strain PSB-4.