Table 2.

Cultivation media

MediumaAmt of component liter−1 (reference)
Mineral salt media
 MinB agarNH4NO3, 1 g; MgSO · 7H2O, 0.5 g; NaCl, 0.5 g; FeSO4 · 7H2O, 0.01 g; K2HPO4, 1 g
 MS agarMineral salt agar (4)
 MSV agarOptimized mineral salt medium (5)
Compost extract mediumCE agarb
Complex media
 SCK agarStarch casein KNO3 agar (14)
 TSB agarTryptic soy broth (Merck), 30 g
 NB agarNutrient broth (Difco), 8 g
 St-II-Soy agarStandard-II-boullion (Merck), 15 g; peptone from soymeal, 5 g; yeast extract, 5 g
 GYM agarStreptomyces medium (3a)
  • a Twenty grams of agar per liter was added to all media. The pH was adjusted to 7.2 ± 0.2. Media were autoclaved for 15 min at 121°C. Mineral salt and compost extract media were used for organism enrichment and the degradation test. Complex media were used for isolation and cultivation of isolated strains.

  • b To make 1 liter of compost extract, 100 g of mature compost (from green wastes) was added to 1 liter of demineralized water and stirred for 24 h at room temperature. The solution was separated from the particles by sieving and centrifugation. The extract had to be autoclaved (20 min, 121°C).