Table 1.

Characteristics of toluene-degrading strains T103 and T104

CharacteristicResult for straina
T103T104
MorphologyRodRod
Flagella
PigmentationYellowYellow
Gram stain++
Acid fast++
Nitrate reduction
Catalase++
Oxidase
Citrate++
Growth on sugars
 Glucose++
 Lactose
 Maltose++
 Sucrose
Growth on aromatic compoundsb
 Toluene++
 Benzene
 Chlorobenzene
o-Xylene++
m-Xylene++
p-Xylene++
 Phenol
  • a +, good growth or activity; −, poor or no growth or activity.

  • b For growth on aromatic compounds, cultures were streaked onto agar plates containing a minimal salts media. Plates were then incubated in desiccators with substrate concentrations of approximately 10 mg/liter. The incubation temperature was 20°C. Each compound was tested twice. ++, very good growth within 3 days; +, good growth after 3 days; −, poor or no growth.