Table 1.

Viruses used in the PCR specificity study, including cyanophages whose DNAs were amplified by cyanophage-specific PCR primers CPS1 and CPS2 and viruses whose DNAs were not amplified by CPS1 and CPS2

VirusHost(s)Area of isolationReference or source
StrainFamilya
Cyanophages amplified by primers CPS1 and CPS2
S-PM2MWH7803, WH8012, WH8018Plymouth, United Kingdom 58
S-BnM1MWH7803Bergen, Norway 56
S-WHM1MWH7803, WH8012Woods Hole, Mass. 58
S-RSM1MWH7803Red Sea, Eilat, Israel 56
S-RSM2MWH7803Red Sea, Eilat, Israel 56
S-BM1MWH7803, WH8012, WH8018Bermuda 58
S-BM3MWH7803, WH8103Sargasso SeaThis study
S-BM4b MWH7803, WH8018BermudaThis study
S-BM5b MWH8018BermudaThis study
S-BM6MWH7803Sargasso SeaThis study
S-MM1MWH7803Miami, Fla. 56
S-MM2MWH7803Miami, Fla. 56
S-MM3MWH7803Miami, Fla. 56
S-MM4MWH7803Miami, Fla. 56
S-MM5MWH7803Miami, Fla. 56
S-MM7MWH7803Miami, Fla. 56
φ2MWH7803, WH8012, WH8018Sargasso Sea 54
φ9MWH7803, WH8012, WH8018Woods Hole, Mass. 54
WH8103
φ14MWH8103Gulf Stream 54
φ33MWH7803Gulf Stream 54
φ34MWH8103Gulf Stream 54
S-PWM1MWH7803Gulf of Mexico 49
S-PWM3MWH7803, SYN48, SNC2, SNC1c Gulf of Mexico 49
Viruses not amplified by primers CPS1 and CPS2
S-BP1PWH8018BermudaThis study
S-BP2PWH8018BermudaThis study
S-BP3PWH7803BermudaThis study
φ12PWH8018Gulf Stream 54
AN-15M Anabaena sp. strain PCC7120Freshwater 27
PW3a-P1M Vibrio natriegens Gulf of Mexico 49
H2/1MH2North Sea 17
H4/4PH4North Sea 17
H54/1SH54North Sea 17
PpV-01Ph Phaeocystis pouchetii Raunefjorden, Norway 28
PpV-02d Ph Phaeocystis pouchetii Raunefjorden, Norway
PpV-04d Ph Phaeocystis pouchetii Raunefjorden, Norway
PpV-05d Ph Phaeocystis pouchetii Raunefjorden, Norway
PpV-06d Ph Phaeocystis pouchetii Raunefjorden, Norway
MpV-Sp1Ph Micromonas pusilla Southern California 16
T4M Escherichia coli Sigma
  • a M, Myoviridae; P,Podoviridae; S, Siphoviridae; Ph,Phycodnaviridae.

  • b Larger products (length, ca. 800 bp) were amplified.

  • c SYN48 and SNC2 are red phycoerythrin-containing strains of a marine Synechococcusspecies, and SNC1 is a green phycocyanin-containing strain of a marineSynechococcus species.

  • d Unpublished results.