Table 1.

Bacterial strains and plasmids used in this study

Strains and plasmidsRelevant genotype and characteristicsReference
E. coli
 JM105 thi rpsL(Strr) endA sbcB15 sbcC hsdR4(rK mK ) Δ(lac-proAB) [F′ traD36 lacI qΔ(lacZ)M15 proA+B+ ] 29
 HB101Smr recA thi pro leu hsdRM+ 14
 MV1190Δ(lac-proAB) Δ(srl-recA)306::Tn10 [F′traD36 proAB lacI qΔ(lacZ)M15] thi supE 27
 MV1190(λ-pir)As MV1190, lysogenized with λ-pirphage 11
P. putida
 KT2442 hsdRRifr 11
 KT2442 (lacI q)As KT2442 with a mini-Tn5-lacI q cassette inserted into the chromosome; Rifr Unpublished
 JB279KT2442 (lacI q) × HB101(RK600) × MV1190(λ-pir)(pJBA29)a; KT2442 (lacI q) with a mini-Tn5-Km-T1-T0-gfpmut3*-PA1/04/03cassette from pJBA29 randomly inserted into the chromosome; Rifr Kmr This study
 JB167KT2442 (lacI q) × HB101(RK600) × MV1190(λ-pir)(pJBA115)a; KT2442 (lacI q) with a mini-Tn5-Km-T1-T0-gfp(LAA)-PA1/04/03cassette from pJBA115 randomly inserted into the chromosome; Rifr Kmr This study
 JB391KT2442 (lacI q) × HB101(RK600) × MV1190(λ-pir)(pJBA117)a; KT2442 (lacI q) with a mini-Tn5-Km-T1-T0-gfp(LVA)-PA1/04/03cassette from pJBA117 randomly inserted into the chromosome; Rifr Kmr This study
 JB340KT2442 (lacI q) × HB101(RK600) × MV1190(λ-pir)(pJBA119)a; KT2442 (lacI q) with a mini-Tn5-Km-T1-T0-gfp(AAV)-PA1/04/03cassette from pJBA119 randomly inserted into the chromosome; Rifr Kmr This study
 JB396KT2442 (lacI q) × HB101(RK600) × MV1190(λ-pir)(pJBA121)a; KT2442 (lacI q) with a mini-Tn5-Km-T1-T0-gfp(ASV)-PA1/04/03-cassette from pJBA121 randomly inserted into the chromosome; RifrKmr This study
Plasmids
 pUC18NotApr; cloning vector 11
 pQE70Apr; donor of RBSII-T0-T1cassette 21
 pUHE24-2Apr; donor of PA1/04/03 promoter 17
 pJBA23Apr; pUC18Not-RBSII-T0-T1 This study
 pJBA24Apr; pUC18Not-PA1/04/03-RBSII-T0-T1 This study
 pJBA27Apr; pUC18Not-PA1/04/03-RBSII-gfpmut3*-T0-T1 This study
 pJBA110Apr; pUC18Not-PA1/04/03-RBSII-gfp(LAA)-T0-T1 This study
 pJBA111Apr; pUC18Not-PA1/04/03-RBSII-gfp(LVA)-T0-T1 This study
 pJBA112Apr; pUC18Not-PA1/04/03-RBSII-gfp(AAV)-T0-T1 This study
 pJBA113Apr; pUC18Not-PA1/04/03-RBSII-gfp(ASV)-T0-T1 This study
 pUT-miniTn5-KmAprKmr; delivery plasmid for mini-Tn5-Km 9
 pJBA28Apr; Kmr; delivery plasmid for mini-Tn5-Km-PA1/04/03-RBSII-gfpmut3*-T0-T1 This study
 pJBA29As pJBA28, but with the PA1/04/03-RBSII-gfpmut3*-T0-T1cassette of pJBA27 in the opposite orientationThis study
 pJBA114Apr Kmr; delivery plasmid for mini-Tn5-Km-PA1/04/03-RBSII-gfp(LAA)-T0-T1 This study
 pJBA115As pJBA114, but with the PA1/04/03-RBSII-gfp(LAA)-T0-T1cassette of pJBA110 in the opposite orientationThis study
 pJBA116Apr Kmr; delivery plasmid for mini-Tn5-Km-PA1/04/03-RBSII-gfp(LVA)-T0-T1 This study
 pJBA117As pJBA116, but with the PA1/04/03-RBSII-gfp(LVA)-T0-T1cassette of pJBA111 in the opposite orientationThis study
 pJBA118Apr Kmr; delivery plasmid for mini-Tn5-Km-PA1/04/03-RBSII-gfp(AAV)-T0-T1 This study
 pJBA119As pJBA118, but with the PA1/04/03-RBSII-gfp(AAV)-T0-T1cassette of pJBA112 in the opposite orientationThis study
 pJBA120Apr Kmr; delivery plasmid for mini-Tn5-Km-PA1/04/03-RBSII-gfp(ASV)-T0-T1 This study
 pJBA121As pJBA120, but with the PA1/04/03-RBSII-gfp(ASV)-T0-T1cassette of pJBA113 in the opposite orientationThis study
 RK600Cmr ori ColE1 RK2-Mob+ RK2-Tra+; helper plasmid in triparental matings 14
  • a Three-factor matings were performed as described previously by Kristensen et al. (16).