Table 1.

Plasmids used in this study

PlasmidRelevant characteristic(s)Reference or source
pUC18Cloning vector, Apr 29
pUC118Cloning vector, Apr 27
pK4 Rhodococcus-E. coli shuttle vector, Kmr 9
pK4HKcospK4 containingcos regionThis study
pVK100Cosmid vector, Kmr Tcr 17
pKHD1pUC118 with 3.0-kb EcoRI fragment of RHA1 carrying etbD1; the direction of etbD1 is identical to that of the lac promoter of pUC118This study
pKHD2pUC118 with 3.0-kb EcoRI fragment of RHA1 carrying etbD1; the direction of etbD1 is opposite that of the lac promoter of pUC118This study
pUAD1pUC18 with 1.2-kb StuI-HindIII fragment of RHA1 carrying bphD This study
pUAD2pUC18 with 1.0-kb ScaI-SphI fragment of RHA1 carryingetbD1 This study
pUAD3pUC18 with 1.3-kb DNA fragment of RHA1 carrying etbD2 This study
pAC1pUC19 with 2.1-kb PstI-SacI fragment of RHA1 carryingbphC 19
pKLA1pK4 with 2.4-kb luciferase structural gene, luxAB from Vibrio harveyi 28
pKLABD1pKLA1 with 1.7-kbBamHI-SacI fragment of RHA1 carrying promoter region of bphD This study
pKLAED1pKLA1 with 1.2-kbEcoRI-XhoI fragment of RHA1 carrying promoter region of etbD1 This study
pKLAED2pKLA1 with 1.2-kbEcoRI fragment of RHA1 carrying promoter region ofetbD2 This study