Table 3.

Relative bactericidal activities of wild-type and mutant MBP-pediocin AcH chimeric proteins

StrainOD600aActivity units/ml of culture brothbProtein levelcCorrected activity units/mld
Wild typee 1.11,0001.01,000
K1N2.2101.44
K11E0.51,0000.82,750
W18R2.3≤101.4≤3
I26T2.41001.629
M31T2.3201.66
A34D0.9≤101.8≤7
N41K1.01001.1100
H42L2.34000.9212
K43N1.81,0001.0611
K43E2.41,0001.1417
  • a Determined for culture broths following a 3-h IPTG induction (average of two experiments).

  • b Calculated by (i) determining the minimum volume (microliters) of culture broth required to produce a zone of growth inhibition in a lawn of L. innocua Lin11 cells and (ii) dividing 1,000 AU · μl/ml by this volume (average of four experiments).

  • c Relative protein synthesis levels were determined by laser scanning densitometry of Coomassie brilliant blue-stained gels containing samples of culture broths (average of two experiments).

  • d Corrected for differences in OD600 values and relative chimeric protein levels in wild-type and mutant culture broths.

  • e Wild-type MBP-pediocin AcH chimeric protein expressed by the E609L/pPR6821 strain.