Table 1.

Bacterial strains and plasmids

Straina or plasmidCharacteristicsReference or source
Strains
Enterococcus faecalisM1Pediocin AcH sensitiveOur isolate
Escherichia coli E609LE609lpp::Tn10, periplasmic leaky Tcr host for malE-papA plasmidsHenry C. Wu
Escherichia coli JM109Host for pMBR1.0-type plasmids 36
Escherichia coliTG1Host used for M13 mutagenesisAmersham
Escherichia coli XL1-BlueHost used for DNA sequencingStratagene
Lactobacillus plantarumNCDO955Pediocin AcH sensitive 3
Leuconostoc mesenteroides LyPediocin AcH sensitiveOur isolate
Listeria innocuaLin11Nonpathogenic, pediocin AcH sensitiveJean Richard, Institut National de la Recherche Agronomique, Paris, France
Pediococcus acidilactici LB42Pediocin AcH sensitiveOur isolate
Pediococcus acidilacticiLB42-923Pediocin AcH producer 26
Plasmids
 pMBR1.0 papABCD operon in pHPS9, Cmr Emr 4
 pMCS9, pMCS14, pMCS24, and pMCS44pMBR1.0 cysteine substitution mutant plasmids, Cmr Emr This study
 pPR682 malE plasmid, Apr New England BioLabs
 pPR6821 malE-papA plasmid, Apr 25
 pKN1, pNK5, and so forth malE-papA substitution mutant plasmids, Apr This study
  • a The wild-type and mutant MBP-pediocin AcH chimeric proteins were tested against 11 other bacterial strains (gram positive and gram negative) that were either sensitive or insensitive to pediocin AcH. None of the mutants acquired activity against resistant strains.